LOCUS       BBa_K176032             1907 bp    DNA     linear       25-Nov-2024
DEFINITION  Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 
            0.70->luxR+pLux/Tet->PoPS
ACCESSION   BBa_K176032
VERSION     BBa_K176032.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_S0101
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     terminator      712..840
                     /label=BBa_B0015
     misc_feature    1..840
                     /label=BBa_K176006
     promoter        849..883
                     /label=BBa_J23101
     misc_feature    896..899
                     /label=conserved
     RBS             892..903
                     /label=BBa_B0034
     misc_feature    910..911
                     /label=prefix
     misc_feature    1083..1083
                     /label=T
     misc_feature    1401..1401
                     /label=A
     CDS             910..1659
                     /label=luxR
     misc_feature    910..1665
                     /label=BBa_C0062
     misc_feature    1666..1690
                     /label=Help:Barcodes
     CDS             910..1665
                     /label=BBa_C0062
     stem_loop       1710..1753
                     /label=stem_loop
     misc_feature    1699..1778
                     /label=BBa_B0010
     terminator      1699..1778
                     /label=BBa_B0010
     stem_loop       1794..1813
                     /label=T7 TE
     misc_feature    1820..1820
                     /label=stop
     polyA_site      1814..1827
                     /label=polya
     misc_feature    1787..1827
                     /label=BBa_B0012
     terminator      1787..1827
                     /label=BBa_B0012
     misc_feature    892..1827
                     /label=BBa_I0462
     misc_feature    849..1827
                     /label=BBa_K176025
     misc_feature    1836..1888
                     /label=R0062
     promoter        1855..1860
                     /label=-35
     promoter        1877..1882
                     /label=-10
     misc_feature    1889..1907
                     /label=tetR
     misc_feature    1888..1888
                     /label=+1
     misc_feature    1836..1855
                     /label=luxR/HSL
     promoter        1836..1907
                     /label=BBa_K176000
     misc_feature    849..1907
                     /label=BBa_K176028
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtt tacagctagc tcagtcctag gtattatgct agctactaga gaaagaggag
      901 aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa
      961 gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat
     1021 tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca
     1081 atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa
     1141 tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt
     1201 gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt
     1261 cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt
     1321 tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac
     1381 ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat
     1441 aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga
     1501 aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat
     1561 ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca
     1621 attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag
     1681 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1801 cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg
     1861 caagaaaatg gtttgttata gtcgaatatc cctatcagtg atagaga
//