LOCUS       BBa_K176034              635 bp    DNA     linear       16-Jun-2024
DEFINITION  PoPS->RBS+lacZalpha-ccdB+T
ACCESSION   BBa_K176034
VERSION     BBa_K176034.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..183
                     /label=a part of lacZalpha (I732006)
     misc_feature    19..21
                     /label=Start
     misc_feature    34..34
                     /label=177bp and a in J32026
     primer_bind     39..55
                     /label=M13 -20 primer
     primer_bind     59..82
                     /label=M13 -47 primer (K176059)
     primer_bind     59..75
                     /label=M13 -40 primer
     misc_feature    183..183
                     /label=g and 69bp in I732006
     misc_feature    190..191
                     /label=at in K145151
     misc_feature    190..495
                     /label=ccdB (K145151)
     misc_feature    273..273
                     /label=c in K145151
     misc_feature    493..498
                     /label=Stop
     primer_bind     206..233
                     /label=VRccdB primer (K176058)
     CDS             19..492
                     /label=lacZalpha-ccdB
     CDS             19..498
                     /label=BBa_K176003
     stem_loop       518..561
                     /label=stem_loop
     misc_feature    507..586
                     /label=BBa_B0010
     terminator      507..586
                     /label=BBa_B0010
     stem_loop       602..621
                     /label=T7 TE
     misc_feature    628..628
                     /label=stop
     polyA_site      622..635
                     /label=polya
     misc_feature    595..635
                     /label=BBa_B0012
     terminator      595..635
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
      241 gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc
      301 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
      361 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
      421 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
      481 ttctggggaa tataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      541 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      601 tggctcacct tcgggtgggc ctttctgcgt ttata
//