BBa_K176061
1
BBa_K176061
PoPS->RBS(weak)+lacZalpha-ccdB->PoPS
2009-08-06T11:00:00Z
2015-05-08T01:11:02Z
B0031 K176003
RBS(weak)+lacZalpha-ccdB
false
false
_282_
0
3908
9
It's complicated
false
weak RBS + lacZalpha-ccdB coding
false
Zongxiao He, Hao Jiang
component2015424
1
BBa_K176003
component2015411
1
BBa_B0031
annotation2015411
1
BBa_B0031
range2015411
1
1
14
annotation2015424
1
BBa_K176003
range2015424
1
21
500
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_K176003
1
lacZa-ccdB
lacZalpha-ccdB coding sequence
2009-07-02T11:00:00Z
2015-07-09T11:37:13Z
PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You).
Forward Primer: GTTTCT TCTAG ATG accatgattacgg attcactggccgtcgttttac
Reverse Primer: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TTATTA tattccccagaacatcaggtta
Digest with XbaI and SpeI, ligate into pSB1A3.
A fusion protein of lacZalpha(I732006) and ccdB(K145151). It should have the function of ccdB to cause E. coli cells to die, but have less toxicity because of the lacZalpha part. It should also have the function of lacZalpha as a reporter for measurement. This part is similar to J32026 but the multiple cloning sites are eliminated. It is constructed by PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You).
true
false
_282_
4206
3908
9
It's complicated
true
The part is similar to BBa_J32026 and the original coding sequence in pluxCcdB3, but the multiple cloning sites are eliminated in order to avoid site-specific mutagenesis. The multiple cloning sites are from lacZalpha in pZErO-2.
After eliminate the MCS, the lacZalpha part is identical to the N-terminal of the wild type gene(BBa_I732006 & BBa_I732005), and fused with the ccdB part (BBa_K145151) with a 3aa linker.
false
Zongxiao He, Hao Jiang
annotation2008006
1
g and 69bp in I732006
range2008006
1
165
165
annotation2008001
1
Start
range2008001
1
1
3
annotation2008003
1
M13 -20 primer
range2008003
1
21
37
annotation2008007
1
at in K145151
range2008007
1
172
173
annotation2011971
1
VRccdB primer (K176058)
range2011971
1
188
215
annotation2008008
1
ccdB (K145151)
range2008008
1
172
477
annotation2008009
1
c in K145151
range2008009
1
255
255
annotation2008000
1
a part of lacZalpha (I732006)
range2008000
1
1
165
annotation2008005
1
M13 -40 primer
range2008005
1
41
57
annotation2008002
1
177bp and a in J32026
range2008002
1
16
16
annotation2020665
1
lacZalpha-ccdB
range2020665
1
1
474
annotation2008004
1
M13 -47 primer (K176059)
range2008004
1
41
64
annotation2008010
1
Stop
range2008010
1
475
480
BBa_K176003_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
BBa_K176061_sequence
1
tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
BBa_B0031_sequence
1
tcacacaggaaacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z