LOCUS       BBa_K176062              463 bp    DNA     linear       24-Nov-2024
DEFINITION  PoPS->RBS(weak)+ccdB+T
ACCESSION   BBa_K176062
VERSION     BBa_K176062.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    21..23
                     /label=start
     misc_feature    324..326
                     /label=stop
     CDS             21..323
                     /label=cds
     CDS             21..326
                     /label=BBa_K145151
     stem_loop       346..389
                     /label=stem_loop
     misc_feature    335..414
                     /label=BBa_B0010
     terminator      335..414
                     /label=BBa_B0010
     stem_loop       430..449
                     /label=T7 TE
     misc_feature    456..456
                     /label=stop
     polyA_site      450..463
                     /label=polya
     misc_feature    423..463
                     /label=BBa_B0012
     terminator      423..463
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aacctactag atgcagttta aggtttacac ctataaaaga gagagccgtt
       61 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
      121 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
      181 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
      241 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
      301 ttaacctgat gttctgggga atataatact agagccaggc atcaaataaa acgaaaggct
      361 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      421 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//