LOCUS       BBa_K176063              637 bp    DNA     linear       03-May-2024
DEFINITION  PoPS->RBS(weak)+lacZalpha-ccdB+T
ACCESSION   BBa_K176063
VERSION     BBa_K176063.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    21..185
                     /label=a part of lacZalpha (I732006)
     misc_feature    21..23
                     /label=Start
     misc_feature    36..36
                     /label=177bp and a in J32026
     primer_bind     41..57
                     /label=M13 -20 primer
     primer_bind     61..84
                     /label=M13 -47 primer (K176059)
     primer_bind     61..77
                     /label=M13 -40 primer
     misc_feature    185..185
                     /label=g and 69bp in I732006
     misc_feature    192..193
                     /label=at in K145151
     misc_feature    192..497
                     /label=ccdB (K145151)
     misc_feature    275..275
                     /label=c in K145151
     misc_feature    495..500
                     /label=Stop
     primer_bind     208..235
                     /label=VRccdB primer (K176058)
     CDS             21..494
                     /label=lacZalpha-ccdB
     CDS             21..500
                     /label=BBa_K176003
     stem_loop       520..563
                     /label=stem_loop
     misc_feature    509..588
                     /label=BBa_B0010
     terminator      509..588
                     /label=BBa_B0010
     stem_loop       604..623
                     /label=T7 TE
     misc_feature    630..630
                     /label=stop
     polyA_site      624..637
                     /label=polya
     misc_feature    597..637
                     /label=BBa_B0012
     terminator      597..637
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aacctactag atgaccatga ttacggattc actggccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      241 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
      301 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
      361 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
      421 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
      481 tgttctgggg aatataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      541 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      601 actggctcac cttcgggtgg gcctttctgc gtttata
//