##gff-version 3
##sequence-region BBa_K176063 1 637
BBa_K176063	.	CDS	21	500	.	+	0	ID=BBa_K176003;Name=BBa_K176003
BBa_K176063	.	primer_binding_site	41	57	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176063	.	point_mutation	275	275	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176063	.	primer_binding_site	61	77	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176063	.	engineered_region	21	185	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176063	.	sequence_alteration	192	193	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176063	.	sequence_alteration	36	36	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176063	.	primer_binding_site	208	235	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176063	.	primer_binding_site	61	84	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176063	.	point_mutation	185	185	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176063	.	CDS	21	494	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176063	.	start_codon	21	23	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176063	.	stop_codon	495	500	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176063	.	engineered_region	192	497	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176063	.	terminator	509	588	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K176063	.	stem_loop	520	563	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176063	.	engineered_region	509	588	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176063	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K176063	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176063	.	terminator	597	637	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K176063	.	stem_loop	604	623	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176063	.	engineered_region	597	637	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176063	.	stop_codon	630	630	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176063	.	polyA_site	624	637	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K176063
tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccc
tggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgat
cgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtc
tgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgct
gtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatg
gccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccatta
acctgatgttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata