LOCUS BBa_K176066 2775 bp DNA linear 22-Nov-2024 DEFINITION aTc&AHL->GFP: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->GFP ACCESSION BBa_K176066 VERSION BBa_K176066.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23101 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..688 /label=tetR CDS 62..688 /label=BBa_I732080 stem_loop 708..751 /label=stem_loop misc_feature 697..776 /label=BBa_B0010 terminator 697..776 /label=BBa_B0010 stem_loop 792..811 /label=T7 TE misc_feature 818..818 /label=stop polyA_site 812..825 /label=polya misc_feature 785..825 /label=BBa_B0012 terminator 785..825 /label=BBa_B0012 misc_feature 44..825 /label=BBa_K176001 promoter 834..868 /label=BBa_J23101 misc_feature 881..884 /label=conserved RBS 877..888 /label=BBa_B0034 misc_feature 895..896 /label=prefix misc_feature 1068..1068 /label=T misc_feature 1386..1386 /label=A CDS 895..1644 /label=luxR misc_feature 895..1650 /label=BBa_C0062 misc_feature 1651..1675 /label=Help:Barcodes CDS 895..1650 /label=BBa_C0062 stem_loop 1695..1738 /label=stem_loop misc_feature 1684..1763 /label=BBa_B0010 terminator 1684..1763 /label=BBa_B0010 stem_loop 1779..1798 /label=T7 TE misc_feature 1805..1805 /label=stop polyA_site 1799..1812 /label=polya misc_feature 1772..1812 /label=BBa_B0012 terminator 1772..1812 /label=BBa_B0012 misc_feature 877..1812 /label=BBa_I0462 misc_feature 834..1812 /label=BBa_K176025 misc_feature 1821..1873 /label=R0062 promoter 1840..1845 /label=-35 promoter 1862..1867 /label=-10 misc_feature 1874..1892 /label=tetR misc_feature 1873..1873 /label=+1 misc_feature 1821..1840 /label=luxR/HSL promoter 1821..1892 /label=BBa_K176000 misc_feature 1905..1908 /label=conserved RBS 1901..1912 /label=BBa_B0034 CDS 1919..2638 /label=GFP protein CDS 1919..2638 /label=BBa_E0040 stem_loop 2658..2701 /label=stem_loop misc_feature 2647..2726 /label=BBa_B0010 terminator 2647..2726 /label=BBa_B0010 stem_loop 2742..2761 /label=T7 TE misc_feature 2768..2768 /label=stop polyA_site 2762..2775 /label=polya misc_feature 2735..2775 /label=BBa_B0012 terminator 2735..2775 /label=BBa_B0012 misc_feature 1821..2775 /label=BBa_K176002 misc_feature 834..2775 /label=BBa_K176026 misc_feature 44..2775 /label=BBa_K176029 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt cctaataata ctagagccag gcatcaaata aaacgaaagg 721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtttacag 841 ctagctcagt cctaggtatt atgctagcta ctagagaaag aggagaaata ctagatgaaa 901 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat 961 aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta 1021 ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac 1081 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta 1141 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta 1201 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt 1261 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa 1321 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt 1381 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat 1441 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat 1501 atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa 1561 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca 1621 attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag atcactacta 1681 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 1741 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 1801 tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg 1861 ttatagtcga atatccctat cagtgataga gatactagag aaagaggaga aatactagat 1921 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 1981 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa 2041 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 2101 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 2161 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa 2221 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa 2281 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt 2341 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat 2401 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 2461 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 2521 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct 2581 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata 2641 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2701 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2761 ctttctgcgt ttata //