##gff-version 3
##sequence-region BBa_K176068 1 1892
BBa_K176068	.	engineered_region	44	1892	.	+	0	ID=BBa_K176031;Name=BBa_K176031
BBa_K176068	.	engineered_region	44	825	.	+	0	ID=BBa_K176001;Name=BBa_K176001;Parent=BBa_K176031
BBa_K176068	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176001
BBa_K176068	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176068	.	terminator	785	825	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176001
BBa_K176068	.	polyA_site	812	825	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176068	.	engineered_region	785	825	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176068	.	stem_loop	792	811	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176068	.	stop_codon	818	818	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176068	.	terminator	697	776	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176001
BBa_K176068	.	stem_loop	708	751	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176068	.	engineered_region	697	776	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176068	.	CDS	62	688	.	+	0	ID=BBa_I732080;Name=BBa_I732080;Parent=BBa_K176001
BBa_K176068	.	CDS	62	688	.	+	0	ID=annotation1940756;Name=tetR;Parent=BBa_I732080
BBa_K176068	.	engineered_region	834	1892	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176031
BBa_K176068	.	promoter	1821	1892	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176068	.	promoter	1862	1867	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176068	.	non_covalent_binding_site	1874	1892	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176068	.	start_codon	1873	1873	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176068	.	non_covalent_binding_site	1821	1840	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176068	.	engineered_region	1821	1873	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176068	.	promoter	1840	1845	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176068	.	engineered_region	834	1812	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176068	.	promoter	834	868	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176068	.	engineered_region	877	1812	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176068	.	CDS	895	1650	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176068	.	CDS	895	1644	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176068	.	engineered_tag	1651	1675	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176068	.	point_mutation	1386	1386	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176068	.	engineered_region	895	896	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176068	.	engineered_region	895	1650	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176068	.	point_mutation	1068	1068	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176068	.	terminator	1684	1763	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176068	.	stem_loop	1695	1738	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176068	.	engineered_region	1684	1763	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176068	.	ribosome_entry_site	877	888	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176068	.	conserved_region	881	884	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176068	.	terminator	1772	1812	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176068	.	polyA_site	1799	1812	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176068	.	engineered_region	1772	1812	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176068	.	stem_loop	1779	1798	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176068	.	stop_codon	1805	1805	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176068	.	promoter	1	35	.	+	0	ID=BBa_K176009;Name=BBa_K176009
BBa_K176068	.	sequence_feature	7	12	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176068	.	promoter	24	29	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176068	.	sequence_feature	18	23	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176068	.	sequence_alteration	17	17	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176068	.	promoter	1	6	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176068	.	sequence_feature	30	35	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
>BBa_K176068
tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagct
actagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagct
tgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcga
tcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatga
tgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatattt
gaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtt
tccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagttt
atttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaat
aataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggata
tttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaa
ccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatag
tgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga