LOCUS       BBa_K176076             1892 bp    DNA     linear       12-May-2024
DEFINITION  aTc&AHL->PoPS: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->PoPS
ACCESSION   BBa_K176076
VERSION     BBa_K176076.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23103
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..688
                     /label=tetR
     CDS             62..688
                     /label=BBa_I732080
     stem_loop       708..751
                     /label=stem_loop
     misc_feature    697..776
                     /label=BBa_B0010
     terminator      697..776
                     /label=BBa_B0010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
     misc_feature    44..825
                     /label=BBa_K176001
     promoter        834..868
                     /label=BBa_J23101
     misc_feature    881..884
                     /label=conserved
     RBS             877..888
                     /label=BBa_B0034
     misc_feature    895..896
                     /label=prefix
     misc_feature    1068..1068
                     /label=T
     misc_feature    1386..1386
                     /label=A
     CDS             895..1644
                     /label=luxR
     misc_feature    895..1650
                     /label=BBa_C0062
     misc_feature    1651..1675
                     /label=Help:Barcodes
     CDS             895..1650
                     /label=BBa_C0062
     stem_loop       1695..1738
                     /label=stem_loop
     misc_feature    1684..1763
                     /label=BBa_B0010
     terminator      1684..1763
                     /label=BBa_B0010
     stem_loop       1779..1798
                     /label=T7 TE
     misc_feature    1805..1805
                     /label=stop
     polyA_site      1799..1812
                     /label=polya
     misc_feature    1772..1812
                     /label=BBa_B0012
     terminator      1772..1812
                     /label=BBa_B0012
     misc_feature    877..1812
                     /label=BBa_I0462
     misc_feature    834..1812
                     /label=BBa_K176025
     misc_feature    1821..1873
                     /label=R0062
     promoter        1840..1845
                     /label=-35
     promoter        1862..1867
                     /label=-10
     misc_feature    1874..1892
                     /label=tetR
     misc_feature    1873..1873
                     /label=+1
     misc_feature    1821..1840
                     /label=luxR/HSL
     promoter        1821..1892
                     /label=BBa_K176000
     misc_feature    834..1892
                     /label=BBa_K176028
     misc_feature    44..1892
                     /label=BBa_K176031
ORIGIN
        1 ctgatagcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt cctaataata ctagagccag gcatcaaata aaacgaaagg
      721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtttacag
      841 ctagctcagt cctaggtatt atgctagcta ctagagaaag aggagaaata ctagatgaaa
      901 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat
      961 aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta
     1021 ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac
     1081 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta
     1141 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta
     1201 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt
     1261 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa
     1321 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt
     1381 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat
     1441 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat
     1501 atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa
     1561 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca
     1621 attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag atcactacta
     1681 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1741 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1801 tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
     1861 ttatagtcga atatccctat cagtgataga ga
//