##gff-version 3 ##sequence-region BBa_K176079 1 2833 BBa_K176079 . promoter 1 35 . + 0 ID=BBa_J23103;Name=BBa_J23103 BBa_K176079 . engineered_region 44 2833 . + 0 ID=BBa_K176030;Name=BBa_K176030 BBa_K176079 . engineered_region 44 883 . + 0 ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176030 BBa_K176079 . terminator 755 883 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006 BBa_K176079 . terminator 843 883 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K176079 . polyA_site 870 883 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K176079 . engineered_region 843 883 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K176079 . stem_loop 850 869 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K176079 . stop_codon 876 876 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K176079 . terminator 755 834 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K176079 . engineered_region 755 834 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K176079 . stem_loop 766 809 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K176079 . engineered_region 44 746 . + 0 ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006 BBa_K176079 . CDS 62 721 . + 0 ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101 BBa_K176079 . engineered_tag 722 746 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_K176079 . CDS 65 681 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_K176079 . tag 682 715 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_K176079 . ribosome_entry_site 44 55 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101 BBa_K176079 . conserved_region 48 51 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K176079 . engineered_region 892 2833 . + 0 ID=BBa_K176026;Name=BBa_K176026;Parent=BBa_K176030 BBa_K176079 . engineered_region 892 1870 . + 0 ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176026 BBa_K176079 . engineered_region 935 1870 . + 0 ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025 BBa_K176079 . terminator 1742 1821 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462 BBa_K176079 . engineered_region 1742 1821 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K176079 . stem_loop 1753 1796 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K176079 . CDS 953 1708 . + 0 ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462 BBa_K176079 . engineered_region 953 1708 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_K176079 . engineered_region 953 954 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_K176079 . CDS 953 1702 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_K176079 . point_mutation 1126 1126 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_K176079 . point_mutation 1444 1444 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_K176079 . engineered_tag 1709 1733 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_K176079 . ribosome_entry_site 935 946 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462 BBa_K176079 . conserved_region 939 942 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K176079 . terminator 1830 1870 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462 BBa_K176079 . polyA_site 1857 1870 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K176079 . engineered_region 1830 1870 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K176079 . stem_loop 1837 1856 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K176079 . stop_codon 1863 1863 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K176079 . promoter 892 926 . + 0 ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025 BBa_K176079 . engineered_region 1879 2833 . + 0 ID=BBa_K176002;Name=BBa_K176002;Parent=BBa_K176026 BBa_K176079 . terminator 2705 2784 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176002 BBa_K176079 . engineered_region 2705 2784 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K176079 . stem_loop 2716 2759 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K176079 . promoter 1879 1950 . + 0 ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176002 BBa_K176079 . promoter 1920 1925 . + 0 ID=annotation2002609;Name=-10;Parent=BBa_K176000 BBa_K176079 . non_covalent_binding_site 1932 1950 . + 0 ID=annotation2002610;Name=tetR;Parent=BBa_K176000 BBa_K176079 . start_codon 1931 1931 . + 0 ID=annotation2002611;Name=+1;Parent=BBa_K176000 BBa_K176079 . non_covalent_binding_site 1879 1898 . + 0 ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000 BBa_K176079 . engineered_region 1879 1931 . + 0 ID=annotation2002607;Name=R0062;Parent=BBa_K176000 BBa_K176079 . promoter 1898 1903 . + 0 ID=annotation2002608;Name=-35;Parent=BBa_K176000 BBa_K176079 . terminator 2793 2833 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176002 BBa_K176079 . polyA_site 2820 2833 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K176079 . engineered_region 2793 2833 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K176079 . stem_loop 2800 2819 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K176079 . stop_codon 2826 2826 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K176079 . ribosome_entry_site 1959 1970 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176002 BBa_K176079 . conserved_region 1963 1966 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K176079 . CDS 1977 2696 . + 0 ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K176002 BBa_K176079 . CDS 1977 2696 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 >BBa_K176079 ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgtccagattagataaa agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc tttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaata ctagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatatt aatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatgg ttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaata tgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaa aaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaaca atggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaa cataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattta accaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgca gtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaa agcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactacta gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacag gtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatacta gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata caaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata