LOCUS       BBa_K176080             1865 bp    DNA     linear       29-Nov-2024
DEFINITION  PoPS*cell density->PoPS: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->P
            oPS
ACCESSION   BBa_K176080
VERSION     BBa_K176080.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    598..629
                     /label=LVA
     CDS             19..597
                     /label=luxI
     misc_feature    19..636
                     /label=BBa_C0061
     misc_feature    637..661
                     /label=Help:Barcodes
     CDS             19..636
                     /label=BBa_C0061
     misc_feature    1..661
                     /label=BBa_C0261
     stem_loop       681..724
                     /label=stem_loop
     misc_feature    670..749
                     /label=BBa_B0010
     terminator      670..749
                     /label=BBa_B0010
     stem_loop       765..784
                     /label=T7 TE
     misc_feature    791..791
                     /label=stop
     polyA_site      785..798
                     /label=polya
     misc_feature    758..798
                     /label=BBa_B0012
     terminator      758..798
                     /label=BBa_B0012
     terminator      670..798
                     /label=BBa_B0015
     misc_feature    1..798
                     /label=BBa_K082014
     promoter        807..841
                     /label=BBa_J23101
     misc_feature    854..857
                     /label=conserved
     RBS             850..861
                     /label=BBa_B0034
     misc_feature    868..869
                     /label=prefix
     misc_feature    1041..1041
                     /label=T
     misc_feature    1359..1359
                     /label=A
     CDS             868..1617
                     /label=luxR
     misc_feature    868..1623
                     /label=BBa_C0062
     misc_feature    1624..1648
                     /label=Help:Barcodes
     CDS             868..1623
                     /label=BBa_C0062
     stem_loop       1668..1711
                     /label=stem_loop
     misc_feature    1657..1736
                     /label=BBa_B0010
     terminator      1657..1736
                     /label=BBa_B0010
     stem_loop       1752..1771
                     /label=T7 TE
     misc_feature    1778..1778
                     /label=stop
     polyA_site      1772..1785
                     /label=polya
     misc_feature    1745..1785
                     /label=BBa_B0012
     terminator      1745..1785
                     /label=BBa_B0012
     misc_feature    850..1785
                     /label=BBa_I0462
     misc_feature    807..1785
                     /label=BBa_K176025
     misc_feature    1794..1846
                     /label=R0062
     promoter        1813..1818
                     /label=-35
     promoter        1835..1840
                     /label=-10
     misc_feature    1847..1865
                     /label=tetR
     misc_feature    1846..1846
                     /label=+1
     misc_feature    1794..1813
                     /label=luxR/HSL
     promoter        1794..1865
                     /label=BBa_K176000
     misc_feature    807..1865
                     /label=BBa_K176028
ORIGIN
        1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      781 ggcctttctg cgtttatata ctagagttta cagctagctc agtcctaggt attatgctag
      841 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga
      901 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat
      961 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg
     1021 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat
     1081 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca
     1141 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa
     1201 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat
     1261 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt
     1321 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa
     1381 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta
     1441 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag
     1501 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc
     1561 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa
     1621 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
     1681 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1741 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta
     1801 ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaatatccc tatcagtgat
     1861 agaga
//