LOCUS       BBa_K176083             1908 bp    DNA     linear       25-Dec-2024
DEFINITION  cell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->P
            oPS
ACCESSION   BBa_K176083
VERSION     BBa_K176083.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    641..672
                     /label=LVA
     CDS             62..640
                     /label=luxI
     misc_feature    62..679
                     /label=BBa_C0061
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             62..679
                     /label=BBa_C0061
     misc_feature    44..704
                     /label=BBa_C0261
     stem_loop       724..767
                     /label=stem_loop
     misc_feature    713..792
                     /label=BBa_B0010
     terminator      713..792
                     /label=BBa_B0010
     stem_loop       808..827
                     /label=T7 TE
     misc_feature    834..834
                     /label=stop
     polyA_site      828..841
                     /label=polya
     misc_feature    801..841
                     /label=BBa_B0012
     terminator      801..841
                     /label=BBa_B0012
     terminator      713..841
                     /label=BBa_B0015
     misc_feature    44..841
                     /label=BBa_K082014
     misc_feature    1..841
                     /label=BBa_K176020
     promoter        850..884
                     /label=BBa_J23101
     misc_feature    897..900
                     /label=conserved
     RBS             893..904
                     /label=BBa_B0034
     misc_feature    911..912
                     /label=prefix
     misc_feature    1084..1084
                     /label=T
     misc_feature    1402..1402
                     /label=A
     CDS             911..1660
                     /label=luxR
     misc_feature    911..1666
                     /label=BBa_C0062
     misc_feature    1667..1691
                     /label=Help:Barcodes
     CDS             911..1666
                     /label=BBa_C0062
     stem_loop       1711..1754
                     /label=stem_loop
     misc_feature    1700..1779
                     /label=BBa_B0010
     terminator      1700..1779
                     /label=BBa_B0010
     stem_loop       1795..1814
                     /label=T7 TE
     misc_feature    1821..1821
                     /label=stop
     polyA_site      1815..1828
                     /label=polya
     misc_feature    1788..1828
                     /label=BBa_B0012
     terminator      1788..1828
                     /label=BBa_B0012
     misc_feature    893..1828
                     /label=BBa_I0462
     misc_feature    850..1828
                     /label=BBa_K176025
     misc_feature    1837..1889
                     /label=R0062
     promoter        1856..1861
                     /label=-35
     promoter        1878..1883
                     /label=-10
     misc_feature    1890..1908
                     /label=tetR
     misc_feature    1889..1889
                     /label=+1
     misc_feature    1837..1856
                     /label=luxR/HSL
     promoter        1837..1908
                     /label=BBa_K176000
     misc_feature    850..1908
                     /label=BBa_K176028
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg
      121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga
      181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga
      241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct
      301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt
      361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga
      421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga
      481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg
      541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc
      601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat
      721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      841 atactagagt ttacagctag ctcagtccta ggtattatgc tagctactag agaaagagga
      901 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa
      961 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca
     1021 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc
     1081 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa
     1141 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt
     1201 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg
     1261 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag
     1321 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa
     1381 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa
     1441 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg
     1501 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca
     1561 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc
     1621 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta
     1681 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1801 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac
     1861 gcaagaaaat ggtttgttat agtcgaatat ccctatcagt gatagaga
//