##gff-version 3
##sequence-region BBa_K176091 1 2510
BBa_K176091	.	engineered_region	1	1865	.	+	0	ID=BBa_K176080;Name=BBa_K176080
BBa_K176091	.	engineered_region	807	1865	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176080
BBa_K176091	.	promoter	1794	1865	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176091	.	promoter	1835	1840	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176091	.	promoter	1813	1818	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176091	.	start_codon	1846	1846	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176091	.	non_covalent_binding_site	1794	1813	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176091	.	non_covalent_binding_site	1847	1865	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176091	.	engineered_region	1794	1846	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176091	.	engineered_region	807	1785	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176091	.	promoter	807	841	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176091	.	engineered_region	850	1785	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176091	.	terminator	1657	1736	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176091	.	stem_loop	1668	1711	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176091	.	engineered_region	1657	1736	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176091	.	ribosome_entry_site	850	861	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176091	.	conserved_region	854	857	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176091	.	CDS	868	1623	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176091	.	engineered_region	868	1623	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176091	.	CDS	868	1617	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176091	.	engineered_region	868	869	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176091	.	point_mutation	1041	1041	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176091	.	engineered_tag	1624	1648	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176091	.	point_mutation	1359	1359	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176091	.	terminator	1745	1785	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176091	.	stop_codon	1778	1778	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176091	.	engineered_region	1745	1785	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176091	.	stem_loop	1752	1771	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176091	.	polyA_site	1772	1785	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176091	.	engineered_region	1	798	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176080
BBa_K176091	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176091	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176091	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176091	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176091	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176091	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176091	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176091	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176091	.	terminator	670	798	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176091	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176091	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176091	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176091	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176091	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176091	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176091	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176091	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176091	.	engineered_region	1874	2510	.	+	0	ID=BBa_K176063;Name=BBa_K176063
BBa_K176091	.	ribosome_entry_site	1874	1887	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176091	.	conserved_region	1880	1883	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176091	.	CDS	1894	2373	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176091	.	point_mutation	2058	2058	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176091	.	engineered_region	1894	2058	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176091	.	primer_binding_site	1914	1930	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176091	.	sequence_alteration	1909	1909	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176091	.	primer_binding_site	2081	2108	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176091	.	CDS	1894	2367	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176091	.	stop_codon	2368	2373	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176091	.	point_mutation	2148	2148	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176091	.	start_codon	1894	1896	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176091	.	engineered_region	2065	2370	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176091	.	sequence_alteration	2065	2066	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176091	.	primer_binding_site	1934	1957	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176091	.	primer_binding_site	1934	1950	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176091	.	terminator	2470	2510	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176091	.	stop_codon	2503	2503	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176091	.	engineered_region	2470	2510	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176091	.	stem_loop	2477	2496	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176091	.	polyA_site	2497	2510	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176091	.	terminator	2382	2461	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176091	.	stem_loop	2393	2436	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176091	.	engineered_region	2382	2461	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K176091
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttac
gcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatg
accatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcc
ttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcag
cctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgat
attattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaac
tttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttat
cggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata