LOCUS BBa_K176093 2553 bp DNA linear 26-Dec-2024 DEFINITION Cell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS) ACCESSION BBa_K176093 VERSION BBa_K176093.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 misc_feature 44..704 /label=BBa_C0261 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 terminator 713..841 /label=BBa_B0015 misc_feature 44..841 /label=BBa_K082014 misc_feature 1..841 /label=BBa_K176019 promoter 850..884 /label=BBa_J23101 misc_feature 897..900 /label=conserved RBS 893..904 /label=BBa_B0034 misc_feature 911..912 /label=prefix misc_feature 1084..1084 /label=T misc_feature 1402..1402 /label=A CDS 911..1660 /label=luxR misc_feature 911..1666 /label=BBa_C0062 misc_feature 1667..1691 /label=Help:Barcodes CDS 911..1666 /label=BBa_C0062 stem_loop 1711..1754 /label=stem_loop misc_feature 1700..1779 /label=BBa_B0010 terminator 1700..1779 /label=BBa_B0010 stem_loop 1795..1814 /label=T7 TE misc_feature 1821..1821 /label=stop polyA_site 1815..1828 /label=polya misc_feature 1788..1828 /label=BBa_B0012 terminator 1788..1828 /label=BBa_B0012 misc_feature 893..1828 /label=BBa_I0462 misc_feature 850..1828 /label=BBa_K176025 misc_feature 1837..1889 /label=R0062 promoter 1856..1861 /label=-35 promoter 1878..1883 /label=-10 misc_feature 1890..1908 /label=tetR misc_feature 1889..1889 /label=+1 misc_feature 1837..1856 /label=luxR/HSL promoter 1837..1908 /label=BBa_K176000 misc_feature 850..1908 /label=BBa_K176028 misc_feature 1..1908 /label=BBa_K176082 misc_feature 1923..1926 /label=conserved RBS 1917..1930 /label=BBa_B0031 misc_feature 1937..2101 /label=a part of lacZalpha (I732006) misc_feature 1937..1939 /label=Start misc_feature 1952..1952 /label=177bp and a in J32026 primer_bind 1957..1973 /label=M13 -20 primer primer_bind 1977..2000 /label=M13 -47 primer (K176059) primer_bind 1977..1993 /label=M13 -40 primer misc_feature 2101..2101 /label=g and 69bp in I732006 misc_feature 2108..2109 /label=at in K145151 misc_feature 2108..2413 /label=ccdB (K145151) misc_feature 2191..2191 /label=c in K145151 misc_feature 2411..2416 /label=Stop primer_bind 2124..2151 /label=VRccdB primer (K176058) CDS 1937..2410 /label=lacZalpha-ccdB CDS 1937..2416 /label=BBa_K176003 stem_loop 2436..2479 /label=stem_loop misc_feature 2425..2504 /label=BBa_B0010 terminator 2425..2504 /label=BBa_B0010 stem_loop 2520..2539 /label=T7 TE misc_feature 2546..2546 /label=stop polyA_site 2540..2553 /label=polya misc_feature 2513..2553 /label=BBa_B0012 terminator 2513..2553 /label=BBa_B0012 misc_feature 1917..2553 /label=BBa_K176063 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagagt ttacagctag ctcagtccta ggtattatgc tagctactag agaaagagga 901 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa 961 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca 1021 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc 1081 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa 1141 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt 1201 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg 1261 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag 1321 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa 1381 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa 1441 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg 1501 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca 1561 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc 1621 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta 1681 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1801 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 1861 gcaagaaaat ggtttgttat agtcgaatat ccctatcagt gatagagata ctagagtcac 1921 acaggaaacc tactagatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg 1981 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 2041 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 2101 atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt 2161 ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag 2221 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga 2281 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga 2341 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt 2401 ctggggaata taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2461 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2521 gctcaccttc gggtgggcct ttctgcgttt ata //