##gff-version 3
##sequence-region BBa_K176095 1 2553
BBa_K176095	.	engineered_region	1917	2553	.	+	0	ID=BBa_K176063;Name=BBa_K176063
BBa_K176095	.	terminator	2513	2553	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176095	.	stop_codon	2546	2546	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176095	.	polyA_site	2540	2553	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176095	.	stem_loop	2520	2539	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176095	.	engineered_region	2513	2553	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176095	.	CDS	1937	2416	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176095	.	stop_codon	2411	2416	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176095	.	sequence_alteration	2108	2109	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176095	.	engineered_region	1937	2101	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176095	.	primer_binding_site	2124	2151	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176095	.	point_mutation	2101	2101	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176095	.	point_mutation	2191	2191	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176095	.	start_codon	1937	1939	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176095	.	primer_binding_site	1977	2000	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176095	.	sequence_alteration	1952	1952	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176095	.	engineered_region	2108	2413	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176095	.	primer_binding_site	1957	1973	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176095	.	primer_binding_site	1977	1993	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176095	.	CDS	1937	2410	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176095	.	terminator	2425	2504	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176095	.	engineered_region	2425	2504	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176095	.	stem_loop	2436	2479	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176095	.	ribosome_entry_site	1917	1930	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176095	.	conserved_region	1923	1926	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176095	.	engineered_region	1	1908	.	+	0	ID=BBa_K176084;Name=BBa_K176084
BBa_K176095	.	engineered_region	850	1908	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176084
BBa_K176095	.	engineered_region	850	1828	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176095	.	promoter	850	884	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176095	.	engineered_region	893	1828	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176095	.	terminator	1788	1828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176095	.	stop_codon	1821	1821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176095	.	polyA_site	1815	1828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176095	.	stem_loop	1795	1814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176095	.	engineered_region	1788	1828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176095	.	CDS	911	1666	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176095	.	engineered_tag	1667	1691	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176095	.	engineered_region	911	1666	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176095	.	engineered_region	911	912	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176095	.	point_mutation	1084	1084	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176095	.	CDS	911	1660	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176095	.	point_mutation	1402	1402	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176095	.	terminator	1700	1779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176095	.	engineered_region	1700	1779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176095	.	stem_loop	1711	1754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176095	.	ribosome_entry_site	893	904	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176095	.	conserved_region	897	900	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176095	.	promoter	1837	1908	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176095	.	promoter	1856	1861	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176095	.	non_covalent_binding_site	1837	1856	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176095	.	promoter	1878	1883	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176095	.	non_covalent_binding_site	1890	1908	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176095	.	start_codon	1889	1889	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176095	.	engineered_region	1837	1889	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176095	.	engineered_region	1	841	.	+	0	ID=BBa_K176021;Name=BBa_K176021;Parent=BBa_K176084
BBa_K176095	.	engineered_region	44	841	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176021
BBa_K176095	.	terminator	713	841	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176095	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176095	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176095	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176095	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176095	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176095	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176095	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176095	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176095	.	engineered_region	44	704	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176095	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176095	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176095	.	CDS	62	679	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176095	.	CDS	62	640	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176095	.	engineered_tag	680	704	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176095	.	engineered_region	62	679	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176095	.	tag	641	672	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176095	.	promoter	1	35	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176021
BBa_K176095	.	promoter	24	29	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176095	.	sequence_feature	18	23	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176095	.	sequence_feature	30	35	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176095	.	sequence_alteration	17	17	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176095	.	sequence_feature	7	12	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176095	.	promoter	1	6	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
>BBa_K176095
tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgactataatgataaaa
aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac
ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg
tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct
gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct
caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat
tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga
gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag
tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcct
aggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataat
taataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaa
tattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaat
ggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaat
taattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctt
atcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagaca
actatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaa
aataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagga
aaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatga
aactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaa
ttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctat
cagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaa
cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaata
gcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataa
aagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatcccc
ctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggc
gcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaa
tgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata