LOCUS       BBa_K176109             1941 bp    DNA     linear       23-Dec-2024
DEFINITION  CI Inverter + GFP Reporter: VR3+pCon 1.00->cI-LVA+pCI->GFP
ACCESSION   BBa_K176109
VERSION     BBa_K176109.1  
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /label=VF3
     misc_feature    1..20
                     /label=BBa_K176037
     promoter        29..63
                     /label=BBa_J23100
     promoter        1..63
                     /label=BBa_K176100
     misc_feature    76..79
                     /label=conserved
     RBS             72..83
                     /label=BBa_B0034
     CDS             93..800
                     /label=cI lambda
     misc_feature    801..833
                     /label=LVA
     misc_feature    840..864
                     /label=Help:Barcodes
     CDS             90..839
                     /label=BBa_C0051
     stem_loop       884..927
                     /label=stem_loop
     misc_feature    873..952
                     /label=BBa_B0010
     terminator      873..952
                     /label=BBa_B0010
     stem_loop       968..987
                     /label=T7 TE
     misc_feature    994..994
                     /label=stop
     polyA_site      988..1001
                     /label=polya
     misc_feature    961..1001
                     /label=BBa_B0012
     terminator      961..1001
                     /label=BBa_B0012
     promoter        1047..1052
                     /label=-10
     promoter        1024..1029
                     /label=-35
     misc_feature    1034..1050
                     /label=OR1
     misc_feature    1010..1026
                     /label=OR2
     misc_feature    1010..1058
                     /label=BBa_R0051
     promoter        1010..1058
                     /label=BBa_R0051
     misc_feature    72..1058
                     /label=BBa_Q04510
     misc_feature    1071..1074
                     /label=conserved
     RBS             1067..1078
                     /label=BBa_B0034
     CDS             1085..1804
                     /label=GFP protein
     CDS             1085..1804
                     /label=BBa_E0040
     stem_loop       1824..1867
                     /label=stem_loop
     misc_feature    1813..1892
                     /label=BBa_B0010
     terminator      1813..1892
                     /label=BBa_B0010
     stem_loop       1908..1927
                     /label=T7 TE
     misc_feature    1934..1934
                     /label=stop
     polyA_site      1928..1941
                     /label=polya
     misc_feature    1901..1941
                     /label=BBa_B0012
     terminator      1901..1941
                     /label=BBa_B0012
     misc_feature    1067..1941
                     /label=BBa_I13504
     misc_feature    72..1941
                     /label=BBa_K176107
ORIGIN
        1 caatacaact ttcggcggca tactagagtt gacggctagc tcagtcctag gtacagtgct
       61 agctactaga gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag
      121 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
      181 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
      241 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
      301 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
      361 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
      421 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
      481 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
      541 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
      601 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
      661 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
      721 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
      781 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac
      841 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc
     1021 gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata
     1081 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1141 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1201 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1261 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1321 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1381 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1441 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1501 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1561 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1621 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1681 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1741 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1801 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1861 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1921 gtgggccttt ctgcgtttat a
//