LOCUS BBa_K176110 1941 bp DNA linear 25-Nov-2024 DEFINITION CI Inverter + GFP Reporter: VR4+pCon 0.70->cI-LVA+pCI->GFP ACCESSION BBa_K176110 VERSION BBa_K176110.1 FEATURES Location/Qualifiers primer_bind 1..20 /label=VR4 misc_feature 1..20 /label=BBa_K176038 promoter 29..63 /label=BBa_J23101 promoter 1..63 /label=BBa_K176101 misc_feature 76..79 /label=conserved RBS 72..83 /label=BBa_B0034 CDS 93..800 /label=cI lambda misc_feature 801..833 /label=LVA misc_feature 840..864 /label=Help:Barcodes CDS 90..839 /label=BBa_C0051 stem_loop 884..927 /label=stem_loop misc_feature 873..952 /label=BBa_B0010 terminator 873..952 /label=BBa_B0010 stem_loop 968..987 /label=T7 TE misc_feature 994..994 /label=stop polyA_site 988..1001 /label=polya misc_feature 961..1001 /label=BBa_B0012 terminator 961..1001 /label=BBa_B0012 promoter 1047..1052 /label=-10 promoter 1024..1029 /label=-35 misc_feature 1034..1050 /label=OR1 misc_feature 1010..1026 /label=OR2 misc_feature 1010..1058 /label=BBa_R0051 promoter 1010..1058 /label=BBa_R0051 misc_feature 72..1058 /label=BBa_Q04510 misc_feature 1071..1074 /label=conserved RBS 1067..1078 /label=BBa_B0034 CDS 1085..1804 /label=GFP protein CDS 1085..1804 /label=BBa_E0040 stem_loop 1824..1867 /label=stem_loop misc_feature 1813..1892 /label=BBa_B0010 terminator 1813..1892 /label=BBa_B0010 stem_loop 1908..1927 /label=T7 TE misc_feature 1934..1934 /label=stop polyA_site 1928..1941 /label=polya misc_feature 1901..1941 /label=BBa_B0012 terminator 1901..1941 /label=BBa_B0012 misc_feature 1067..1941 /label=BBa_I13504 misc_feature 72..1941 /label=BBa_K176107 ORIGIN 1 ggtttggtgc gtgttgttag tactagagtt tacagctagc tcagtcctag gtattatgct 61 agctactaga gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag 121 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg 181 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt 241 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca 301 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag 361 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc 421 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg 481 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca 541 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc 601 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta 661 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt 721 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt 781 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac 841 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca 901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc 1021 gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata 1081 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 1141 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 1201 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 1261 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 1321 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 1381 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 1441 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 1501 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 1561 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 1621 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 1681 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 1741 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 1801 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 1861 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 1921 gtgggccttt ctgcgtttat a //