LOCUS BBa_K176115 2336 bp DNA linear 26-Dec-2024 DEFINITION Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->c cdB(weak RBS) ACCESSION BBa_K176115 VERSION BBa_K176115.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 misc_feature 1..661 /label=BBa_C0261 stem_loop 681..724 /label=stem_loop misc_feature 670..749 /label=BBa_B0010 terminator 670..749 /label=BBa_B0010 stem_loop 765..784 /label=T7 TE misc_feature 791..791 /label=stop polyA_site 785..798 /label=polya misc_feature 758..798 /label=BBa_B0012 terminator 758..798 /label=BBa_B0012 terminator 670..798 /label=BBa_B0015 misc_feature 1..798 /label=BBa_K082014 promoter 807..841 /label=BBa_J23101 misc_feature 854..857 /label=conserved RBS 850..861 /label=BBa_B0034 misc_feature 868..869 /label=prefix misc_feature 1041..1041 /label=T misc_feature 1359..1359 /label=A CDS 868..1617 /label=luxR misc_feature 868..1623 /label=BBa_C0062 misc_feature 1624..1648 /label=Help:Barcodes CDS 868..1623 /label=BBa_C0062 stem_loop 1668..1711 /label=stem_loop misc_feature 1657..1736 /label=BBa_B0010 terminator 1657..1736 /label=BBa_B0010 stem_loop 1752..1771 /label=T7 TE misc_feature 1778..1778 /label=stop polyA_site 1772..1785 /label=polya misc_feature 1745..1785 /label=BBa_B0012 terminator 1745..1785 /label=BBa_B0012 misc_feature 850..1785 /label=BBa_I0462 misc_feature 807..1785 /label=BBa_K176025 misc_feature 1794..1846 /label=R0062 promoter 1813..1818 /label=-35 promoter 1835..1840 /label=-10 misc_feature 1847..1865 /label=tetR misc_feature 1846..1846 /label=+1 misc_feature 1794..1813 /label=luxR/HSL promoter 1794..1865 /label=BBa_K176000 misc_feature 807..1865 /label=BBa_K176028 misc_feature 1..1865 /label=BBa_K176080 misc_feature 1880..1883 /label=conserved RBS 1874..1887 /label=BBa_B0031 misc_feature 1894..1896 /label=start misc_feature 2197..2199 /label=stop CDS 1894..2196 /label=cds CDS 1894..2199 /label=BBa_K145151 stem_loop 2219..2262 /label=stem_loop misc_feature 2208..2287 /label=BBa_B0010 terminator 2208..2287 /label=BBa_B0010 stem_loop 2303..2322 /label=T7 TE misc_feature 2329..2329 /label=stop polyA_site 2323..2336 /label=polya misc_feature 2296..2336 /label=BBa_B0012 terminator 2296..2336 /label=BBa_B0012 misc_feature 1874..2336 /label=BBa_K176062 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 781 ggcctttctg cgtttatata ctagagttta cagctagctc agtcctaggt attatgctag 841 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 901 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 961 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 1021 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 1081 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 1141 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 1201 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 1261 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 1321 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 1381 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 1441 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 1501 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 1561 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 1621 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 1681 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1741 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta 1801 ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaatatccc tatcagtgat 1861 agagatacta gagtcacaca ggaaacctac tagatgcagt ttaaggttta cacctataaa 1921 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg 1981 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa 2041 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc 2101 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac 2161 atcaaaaacg ccattaacct gatgttctgg ggaatataat actagagcca ggcatcaaat 2221 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2281 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //