LOCUS       BBa_K176124             1899 bp    DNA     linear       27-Sep-2024
DEFINITION  TetR repressible AHL->GFP Receiver (PoPS->luxR): 
            PoPS->luxR+pLux/Tet->GFP
ACCESSION   BBa_K176124
VERSION     BBa_K176124.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     misc_feature    1..936
                     /label=BBa_I0462
     misc_feature    945..997
                     /label=R0062
     promoter        964..969
                     /label=-35
     promoter        986..991
                     /label=-10
     misc_feature    998..1016
                     /label=tetR
     misc_feature    997..997
                     /label=+1
     misc_feature    945..964
                     /label=luxR/HSL
     promoter        945..1016
                     /label=BBa_K176000
     misc_feature    1029..1032
                     /label=conserved
     RBS             1025..1036
                     /label=BBa_B0034
     CDS             1043..1762
                     /label=GFP protein
     CDS             1043..1762
                     /label=BBa_E0040
     stem_loop       1782..1825
                     /label=stem_loop
     misc_feature    1771..1850
                     /label=BBa_B0010
     terminator      1771..1850
                     /label=BBa_B0010
     stem_loop       1866..1885
                     /label=T7 TE
     misc_feature    1892..1892
                     /label=stop
     polyA_site      1886..1899
                     /label=polya
     misc_feature    1859..1899
                     /label=BBa_B0012
     terminator      1859..1899
                     /label=BBa_B0012
     misc_feature    945..1899
                     /label=BBa_K176002
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
      961 aggtttacgc aagaaaatgg tttgttatag tcgaatatcc ctatcagtga tagagatact
     1021 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
     1081 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1141 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1201 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     1261 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     1321 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     1381 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     1441 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     1501 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     1561 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1621 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1681 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1741 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1801 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1861 acactggctc accttcgggt gggcctttct gcgtttata
//