LOCUS       BBa_K176128             1942 bp    DNA     linear       15-Nov-2024
DEFINITION  TetR repressible AHL->GFP Receiver (Medium LuxR): pCon 
            0.15->luxR+pLux/Tet->GFP
ACCESSION   BBa_K176128
VERSION     BBa_K176128.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     misc_feature    17..17
                     /label=c in J23115
     misc_feature    21..21
                     /label=t in J23115
     misc_feature    7..12
                     /label=NheI
     misc_feature    30..35
                     /label=NheI
     misc_feature    18..23
                     /label=AvrII
     promoter        1..35
                     /label=BBa_K176008
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    988..1040
                     /label=R0062
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1041..1059
                     /label=tetR
     misc_feature    1040..1040
                     /label=+1
     misc_feature    988..1007
                     /label=luxR/HSL
     promoter        988..1059
                     /label=BBa_K176000
     misc_feature    1072..1075
                     /label=conserved
     RBS             1068..1079
                     /label=BBa_B0034
     CDS             1086..1805
                     /label=GFP protein
     CDS             1086..1805
                     /label=BBa_E0040
     stem_loop       1825..1868
                     /label=stem_loop
     misc_feature    1814..1893
                     /label=BBa_B0010
     terminator      1814..1893
                     /label=BBa_B0010
     stem_loop       1909..1928
                     /label=T7 TE
     misc_feature    1935..1935
                     /label=stop
     polyA_site      1929..1942
                     /label=polya
     misc_feature    1902..1942
                     /label=BBa_B0012
     terminator      1902..1942
                     /label=BBa_B0012
     misc_feature    988..1942
                     /label=BBa_K176002
     misc_feature    44..1942
                     /label=BBa_K176124
ORIGIN
        1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat
     1081 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     1141 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     1201 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
     1261 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
     1321 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     1381 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     1441 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     1501 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     1561 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
     1621 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     1681 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     1741 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     1801 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1921 ggtgggcctt tctgcgttta ta
//