##gff-version 3
##sequence-region BBa_K176131 1 1530
BBa_K176131	.	engineered_region	1068	1530	.	+	0	ID=BBa_K176062;Name=BBa_K176062
BBa_K176131	.	terminator	1490	1530	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176062
BBa_K176131	.	stem_loop	1497	1516	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176131	.	polyA_site	1517	1530	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176131	.	stop_codon	1523	1523	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176131	.	engineered_region	1490	1530	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176131	.	ribosome_entry_site	1068	1081	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176062
BBa_K176131	.	conserved_region	1074	1077	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176131	.	CDS	1088	1393	.	+	0	ID=BBa_K145151;Name=BBa_K145151;Parent=BBa_K176062
BBa_K176131	.	stop_codon	1391	1393	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K176131	.	start_codon	1088	1090	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K176131	.	CDS	1088	1390	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K176131	.	terminator	1402	1481	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176062
BBa_K176131	.	stem_loop	1413	1456	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176131	.	engineered_region	1402	1481	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176131	.	engineered_region	1	1059	.	+	0	ID=BBa_K176125;Name=BBa_K176125
BBa_K176131	.	promoter	1	35	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176125
BBa_K176131	.	promoter	24	29	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176131	.	sequence_feature	30	35	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176131	.	sequence_alteration	17	17	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176131	.	sequence_feature	7	12	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176131	.	sequence_feature	18	23	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176131	.	promoter	1	6	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176131	.	engineered_region	44	1059	.	+	0	ID=BBa_K176123;Name=BBa_K176123;Parent=BBa_K176125
BBa_K176131	.	promoter	988	1059	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176123
BBa_K176131	.	promoter	1029	1034	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176131	.	promoter	1007	1012	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176131	.	start_codon	1040	1040	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176131	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176131	.	non_covalent_binding_site	1041	1059	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176131	.	engineered_region	988	1040	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176131	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176123
BBa_K176131	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176131	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176131	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176131	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176131	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176131	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176131	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176131	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176131	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176131	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176131	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176131	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176131	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176131	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176131	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176131	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176131	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
>BBa_K176131
tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgcagtttaaggtttaca
cctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggt
gatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaa
agctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccacc
gcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata