##gff-version 3
##sequence-region BBa_K176132 1 1704
BBa_K176132	.	engineered_region	1	1059	.	+	0	ID=BBa_K176125;Name=BBa_K176125
BBa_K176132	.	engineered_region	44	1059	.	+	0	ID=BBa_K176123;Name=BBa_K176123;Parent=BBa_K176125
BBa_K176132	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176123
BBa_K176132	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176132	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176132	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176132	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176132	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176132	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176132	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176132	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176132	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176132	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176132	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176132	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176132	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176132	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176132	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176132	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176132	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176132	.	promoter	988	1059	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176123
BBa_K176132	.	promoter	1029	1034	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176132	.	promoter	1007	1012	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176132	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176132	.	start_codon	1040	1040	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176132	.	non_covalent_binding_site	1041	1059	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176132	.	engineered_region	988	1040	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176132	.	promoter	1	35	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176125
BBa_K176132	.	sequence_alteration	17	17	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176132	.	promoter	24	29	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176132	.	sequence_feature	18	23	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176132	.	sequence_feature	7	12	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176132	.	promoter	1	6	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176132	.	sequence_feature	30	35	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176132	.	engineered_region	1068	1704	.	+	0	ID=BBa_K176063;Name=BBa_K176063
BBa_K176132	.	ribosome_entry_site	1068	1081	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176132	.	conserved_region	1074	1077	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176132	.	terminator	1576	1655	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176132	.	stem_loop	1587	1630	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176132	.	engineered_region	1576	1655	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176132	.	terminator	1664	1704	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176132	.	polyA_site	1691	1704	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176132	.	engineered_region	1664	1704	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176132	.	stem_loop	1671	1690	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176132	.	stop_codon	1697	1697	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176132	.	CDS	1088	1567	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176132	.	point_mutation	1342	1342	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176132	.	primer_binding_site	1275	1302	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176132	.	primer_binding_site	1128	1144	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176132	.	sequence_alteration	1259	1260	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176132	.	stop_codon	1562	1567	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176132	.	primer_binding_site	1128	1151	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176132	.	engineered_region	1088	1252	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176132	.	point_mutation	1252	1252	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176132	.	CDS	1088	1561	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176132	.	engineered_region	1259	1564	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176132	.	primer_binding_site	1108	1124	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176132	.	sequence_alteration	1103	1103	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176132	.	start_codon	1088	1090	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
>BBa_K176132
tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggatt
cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccc
tttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcag
tttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccgg
ggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgca
tatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggct
gatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata