aTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
Input aTc activates PoPS. Input AHL activates PoPS.
Input1: aTc Input2: AHL Output: PoPS Design: pCon 0.70(J23101) generates TetR-LVA pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc
Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
Repressible AHL->PoPS Receiver Input PoPS represses PoPS. Input AHL activates PoPS.
Input1: PoPS Input2: AHL Output: PoPS Design: Input1 PoPS generates TetR-LVA pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates Output PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc
TetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPS
AHL -> PoPS Receiver TetR repressible High LuxR level Similar to F2620, but use pLux/Tet hybrid promoter.
Input: AHL Output: PoPS Design: pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates Output PoPS, activated by LuxR and Input AHL, repressed by TetR and no aTc
Double terminator consisting of BBa_B0010 and BBa_B0012
constitutive promoter family member J23107 actual sequence (pCon 0.36)
The sequence of J23107 is wrong according to sequencing result of the registry and ours. This part K176009 is created for the actual promoter. http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=3628 http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=1184 J23107: tttacggctagctcagCcctaggtattatgctagc K176009: tttacggctagctcagTcctaggtattatgctagc
TetR repressible AHL->PoPS Receiver (PoPS->luxR): PoPS->luxR+pLux/Tet->PoPS
AHL -> PoPS Receiver TetR repressible Similar to F2620, but use pLux/Tet hybrid promoter. Different input PoPS result different luxR level, K176028, K176125, K176127, K176129.
Input1: PoPS Input2: AHL Output: PoPS Design: Input1 PoPS generates LuxR pLux/Tet(K176000) generates PoPS output, activated by LuxR and Input2 AHL, repressed by TetR and no aTc
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
tetracycline repressor from transposon Tn10 (+LVA)
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
sense TWO INPUTS, activation by AHL and repression by tetR
V. fischeri <genbank>AF170104</genbank>
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Conversion of the iGEM parts registry to SBOL2.1