BBa_K176137
type: ComponentDefinition
persistent identity: BBa K176137
display id: BBa_K176137
version: 1
was generated by: iGEM to SBOL conversion
description: aTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
created: 2009-10-06T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176009 K176133
owned by: james
owned by: myers
mutable description: Input aTc activates PoPS. Input AHL activates PoPS.
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Input1: aTc Input2: AHL Output: PoPS Design: pCon 0.36(K176009) generates TetR-LVA pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K176133
component: BBa_K176009
sequence annotation: BBa_K176009
sequence annotation: BBa_K176133
sequence: https://synbiohub.org/public/igem/BBa_K176137_sequence/1

BBa_K176133
type: Component
persistent identity: component2293692
display id: component2293692
version: 1
top level: BBa_K176137
definition: BBa_K176133
access: public

BBa_K176009
type: Component
persistent identity: component2293645
display id: component2293645
version: 1
top level: BBa_K176137
definition: pCon 0.36
access: public

BBa_K176009
type: SequenceAnnotation
persistent identity: annotation2293645
display id: annotation2293645
version: 1
top level: BBa_K176137
location: https://synbiohub.org/public/igem/BBa_K176137/annotation2293645/range2293645/1
component: BBa_K176009

https://synbiohub.org/public/igem/BBa_K176137/annotation2293645/range2293645/1
type: Range
persistent identity: range2293645
display id: range2293645
version: 1
top level: BBa_K176137
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K176133
type: SequenceAnnotation
persistent identity: annotation2293692
display id: annotation2293692
version: 1
top level: BBa_K176137
location: https://synbiohub.org/public/igem/BBa_K176137/annotation2293692/range2293692/1
component: BBa_K176133

https://synbiohub.org/public/igem/BBa_K176137/annotation2293692/range2293692/1
type: Range
persistent identity: range2293692
display id: range2293692
version: 1
top level: BBa_K176137
direction: direction/reverse
start: 44
end: 1950
orientation: inline

BBa_K176133
type: ComponentDefinition
persistent identity: BBa K176133
display id: BBa_K176133
version: 1
was generated by: iGEM to SBOL conversion
description: Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
created: 2009-10-06T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176006 K176125
owned by: james
owned by: myers
mutable description: Repressible AHL->PoPS Receiver Input PoPS represses PoPS. Input AHL activates PoPS.
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Input1: PoPS Input2: AHL Output: PoPS Design: Input1 PoPS generates TetR-LVA pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates Output PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K176125
component: BBa_K176006
sequence annotation: BBa_K176006
sequence annotation: BBa_K176125
sequence: https://synbiohub.org/public/igem/BBa_K176133_sequence/1

BBa_K176125
type: Component
persistent identity: component2287416
display id: component2287416
version: 1
top level: BBa_K176133
definition: BBa_K176125
access: public

BBa_K176006
type: Component
persistent identity: component2287385
display id: component2287385
version: 1
top level: BBa_K176133
definition: BBa_K176006
access: public

BBa_K176006
type: SequenceAnnotation
persistent identity: annotation2287385
display id: annotation2287385
version: 1
top level: BBa_K176133
location: https://synbiohub.org/public/igem/BBa_K176133/annotation2287385/range2287385/1
component: BBa_K176006

https://synbiohub.org/public/igem/BBa_K176133/annotation2287385/range2287385/1
type: Range
persistent identity: range2287385
display id: range2287385
version: 1
top level: BBa_K176133
direction: direction/reverse
start: 1
end: 840
orientation: inline

BBa_K176125
type: SequenceAnnotation
persistent identity: annotation2287416
display id: annotation2287416
version: 1
top level: BBa_K176133
location: https://synbiohub.org/public/igem/BBa_K176133/annotation2287416/range2287416/1
component: BBa_K176125

https://synbiohub.org/public/igem/BBa_K176133/annotation2287416/range2287416/1
type: Range
persistent identity: range2287416
display id: range2287416
version: 1
top level: BBa_K176133
direction: direction/reverse
start: 849
end: 1907
orientation: inline

pCon 0.36
type: ComponentDefinition
persistent identity: BBa K176009
display id: BBa_K176009
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member J23107 actual sequence (pCon 0.36)
created: 2009-06-19T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: This is the actual sequence of J23107.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The sequence of J23107 is wrong according to sequencing result of the registry and ours. This part K176009 is created for the actual promoter. http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=3628 http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=1184 J23107: tttacggctagctcagCcctaggtattatgctagc K176009: tttacggctagctcagTcctaggtattatgctagc
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: J23107
star: false
creator: Hao Jiang, Danqian Liu, Chao Li
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: NheI
sequence annotation: c in J23107
sequence annotation: AvrII
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K176009_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2005855
display id: annotation2005855
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005855/range2005855/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176009/annotation2005855/range2005855/1
type: Range
persistent identity: range2005855
display id: range2005855
version: 1
top level: pCon 0.36
direction: direction/forward
start: 1
end: 6
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2005856
display id: annotation2005856
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005856/range2005856/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005856/range2005856/1
type: Range
persistent identity: range2005856
display id: range2005856
version: 1
top level: pCon 0.36
direction: direction/forward
start: 7
end: 12
orientation: inline

c in J23107
type: SequenceAnnotation
persistent identity: annotation2005857
display id: annotation2005857
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005857/range2005857/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K176009/annotation2005857/range2005857/1
type: Range
persistent identity: range2005857
display id: range2005857
version: 1
top level: pCon 0.36
direction: direction/forward
start: 17
end: 17
orientation: inline

AvrII
type: SequenceAnnotation
persistent identity: annotation2005858
display id: annotation2005858
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005858/range2005858/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005858/range2005858/1
type: Range
persistent identity: range2005858
display id: range2005858
version: 1
top level: pCon 0.36
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2005859
display id: annotation2005859
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005859/range2005859/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K176009/annotation2005859/range2005859/1
type: Range
persistent identity: range2005859
display id: range2005859
version: 1
top level: pCon 0.36
direction: direction/forward
start: 24
end: 29
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2005860
display id: annotation2005860
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005860/range2005860/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005860/range2005860/1
type: Range
persistent identity: range2005860
display id: range2005860
version: 1
top level: pCon 0.36
direction: direction/forward
start: 30
end: 35
orientation: inline

BBa_K176006
type: ComponentDefinition
persistent identity: BBa K176006
display id: BBa_K176006
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->RBS+tetR-LVA+T
created: 2009-06-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: S0101 B0015
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS->tetR(with LVA)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3971
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Generate tetR(with LVA)
star: false
creator: Chao Li,Danqian Liu,Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_S0101
sequence annotation: BBa_S0101
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K176006_sequence/1

BBa_B0015
type: Component
persistent identity: component2249512
display id: component2249512
version: 1
top level: BBa_K176006
definition: BBa_B0015
access: public

BBa_S0101
type: Component
persistent identity: component2249505
display id: component2249505
version: 1
top level: BBa_K176006
definition: BBa_S0101
access: public

BBa_S0101
type: SequenceAnnotation
persistent identity: annotation2249505
display id: annotation2249505
version: 1
top level: BBa_K176006
location: https://synbiohub.org/public/igem/BBa_K176006/annotation2249505/range2249505/1
component: BBa_S0101

https://synbiohub.org/public/igem/BBa_K176006/annotation2249505/range2249505/1
type: Range
persistent identity: range2249505
display id: range2249505
version: 1
top level: BBa_K176006
direction: direction/reverse
start: 1
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249512
display id: annotation2249512
version: 1
top level: BBa_K176006
location: https://synbiohub.org/public/igem/BBa_K176006/annotation2249512/range2249512/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K176006/annotation2249512/range2249512/1
type: Range
persistent identity: range2249512
display id: range2249512
version: 1
top level: BBa_K176006
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_K176125
type: ComponentDefinition
persistent identity: BBa K176125
display id: BBa_K176125
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPS
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176009 K176123
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible High LuxR level Similar to F2620, but use pLux/Tet hybrid promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Input: AHL Output: PoPS Design: pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates Output PoPS, activated by LuxR and Input AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K176123
component: BBa_K176009
sequence annotation: BBa_K176009
sequence annotation: BBa_K176123
sequence: https://synbiohub.org/public/igem/BBa_K176125_sequence/1

BBa_K176123
type: Component
persistent identity: component2262819
display id: component2262819
version: 1
top level: BBa_K176125
definition: BBa_K176123
access: public

BBa_K176009
type: Component
persistent identity: component2262796
display id: component2262796
version: 1
top level: BBa_K176125
definition: pCon 0.36
access: public

BBa_K176009
type: SequenceAnnotation
persistent identity: annotation2262796
display id: annotation2262796
version: 1
top level: BBa_K176125
location: https://synbiohub.org/public/igem/BBa_K176125/annotation2262796/range2262796/1
component: BBa_K176009

https://synbiohub.org/public/igem/BBa_K176125/annotation2262796/range2262796/1
type: Range
persistent identity: range2262796
display id: range2262796
version: 1
top level: BBa_K176125
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K176123
type: SequenceAnnotation
persistent identity: annotation2262819
display id: annotation2262819
version: 1
top level: BBa_K176125
location: https://synbiohub.org/public/igem/BBa_K176125/annotation2262819/range2262819/1
component: BBa_K176123

https://synbiohub.org/public/igem/BBa_K176125/annotation2262819/range2262819/1
type: Range
persistent identity: range2262819
display id: range2262819
version: 1
top level: BBa_K176125
direction: direction/reverse
start: 44
end: 1059
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S0101
type: ComponentDefinition
persistent identity: BBa S0101
display id: BBa_S0101
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0040
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S0101_sequence/1

BBa_C0040
type: Component
persistent identity: component939723
display id: component939723
version: 1
top level: BBa_S0101
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component939713
display id: component939713
version: 1
top level: BBa_S0101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939713
display id: annotation939713
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
type: Range
persistent identity: range939713
display id: range939713
version: 1
top level: BBa_S0101
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939723
display id: annotation939723
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
type: Range
persistent identity: range939723
display id: range939723
version: 1
top level: BBa_S0101
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_K176123
type: ComponentDefinition
persistent identity: BBa K176123
display id: BBa_K176123
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (PoPS->luxR): PoPS->luxR+pLux/Tet->PoPS
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: I0462 K176000
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible Similar to F2620, but use pLux/Tet hybrid promoter. Different input PoPS result different luxR level, K176028, K176125, K176127, K176129.
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Input1: PoPS Input2: AHL Output: PoPS Design: Input1 PoPS generates LuxR pLux/Tet(K176000) generates PoPS output, activated by LuxR and Input2 AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_I0462
component: BBa_K176000
sequence annotation: BBa_I0462
sequence annotation: BBa_K176000
sequence: https://synbiohub.org/public/igem/BBa_K176123_sequence/1

BBa_I0462
type: Component
persistent identity: component2255854
display id: component2255854
version: 1
top level: BBa_K176123
definition: LuxR
access: public

BBa_K176000
type: Component
persistent identity: component2255861
display id: component2255861
version: 1
top level: BBa_K176123
definition: pLux/Tet
access: public

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2255854
display id: annotation2255854
version: 1
top level: BBa_K176123
location: https://synbiohub.org/public/igem/BBa_K176123/annotation2255854/range2255854/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K176123/annotation2255854/range2255854/1
type: Range
persistent identity: range2255854
display id: range2255854
version: 1
top level: BBa_K176123
direction: direction/reverse
start: 1
end: 936
orientation: inline

BBa_K176000
type: SequenceAnnotation
persistent identity: annotation2255861
display id: annotation2255861
version: 1
top level: BBa_K176123
location: https://synbiohub.org/public/igem/BBa_K176123/annotation2255861/range2255861/1
component: BBa_K176000

https://synbiohub.org/public/igem/BBa_K176123/annotation2255861/range2255861/1
type: Range
persistent identity: range2255861
display id: range2255861
version: 1
top level: BBa_K176123
direction: direction/reverse
start: 945
end: 1016
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K176137_sequence/1
type: Sequence
persistent identity: BBa K176137 sequence
display id: BBa_K176137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176133_sequence/1
type: Sequence
persistent identity: BBa K176133 sequence
display id: BBa_K176133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176009_sequence/1
type: Sequence
persistent identity: BBa K176009 sequence
display id: BBa_K176009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176006_sequence/1
type: Sequence
persistent identity: BBa K176006 sequence
display id: BBa_K176006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176125_sequence/1
type: Sequence
persistent identity: BBa K176125 sequence
display id: BBa_K176125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0101_sequence/1
type: Sequence
persistent identity: BBa S0101 sequence
display id: BBa_S0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176123_sequence/1
type: Sequence
persistent identity: BBa K176123 sequence
display id: BBa_K176123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z