LOCUS BBa_K176140 2833 bp DNA linear 22-Nov-2024 DEFINITION aTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP ACCESSION BBa_K176140 VERSION BBa_K176140.1 FEATURES Location/Qualifiers promoter 1..6 /label=-35 promoter 24..29 /label=-10 misc_feature 17..17 /label=c in J23115 misc_feature 21..21 /label=t in J23115 misc_feature 7..12 /label=NheI misc_feature 30..35 /label=NheI misc_feature 18..23 /label=AvrII promoter 1..35 /label=BBa_K176008 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 misc_feature 44..746 /label=BBa_S0101 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 44..883 /label=BBa_K176006 promoter 892..897 /label=-35 misc_feature 898..903 /label=NheI misc_feature 908..908 /label=c in J23107 misc_feature 909..914 /label=AvrII promoter 915..920 /label=-10 misc_feature 921..926 /label=NheI promoter 892..926 /label=BBa_K176009 misc_feature 939..942 /label=conserved RBS 935..946 /label=BBa_B0034 misc_feature 953..954 /label=prefix misc_feature 1126..1126 /label=T misc_feature 1444..1444 /label=A CDS 953..1702 /label=luxR misc_feature 953..1708 /label=BBa_C0062 misc_feature 1709..1733 /label=Help:Barcodes CDS 953..1708 /label=BBa_C0062 stem_loop 1753..1796 /label=stem_loop misc_feature 1742..1821 /label=BBa_B0010 terminator 1742..1821 /label=BBa_B0010 stem_loop 1837..1856 /label=T7 TE misc_feature 1863..1863 /label=stop polyA_site 1857..1870 /label=polya misc_feature 1830..1870 /label=BBa_B0012 terminator 1830..1870 /label=BBa_B0012 misc_feature 935..1870 /label=BBa_I0462 misc_feature 1879..1931 /label=R0062 promoter 1898..1903 /label=-35 promoter 1920..1925 /label=-10 misc_feature 1932..1950 /label=tetR misc_feature 1931..1931 /label=+1 misc_feature 1879..1898 /label=luxR/HSL promoter 1879..1950 /label=BBa_K176000 misc_feature 1963..1966 /label=conserved RBS 1959..1970 /label=BBa_B0034 CDS 1977..2696 /label=GFP protein CDS 1977..2696 /label=BBa_E0040 stem_loop 2716..2759 /label=stem_loop misc_feature 2705..2784 /label=BBa_B0010 terminator 2705..2784 /label=BBa_B0010 stem_loop 2800..2819 /label=T7 TE misc_feature 2826..2826 /label=stop polyA_site 2820..2833 /label=polya misc_feature 2793..2833 /label=BBa_B0012 terminator 2793..2833 /label=BBa_B0012 misc_feature 1879..2833 /label=BBa_K176002 misc_feature 935..2833 /label=BBa_K176124 misc_feature 892..2833 /label=BBa_K176126 misc_feature 44..2833 /label=BBa_K176134 ORIGIN 1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtttacggct 901 agctcagtcc taggtattat gctagctact agagaaagag gagaaatact agatgaaaaa 961 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa 1021 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact 1081 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc 1141 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga 1201 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa 1261 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag 1321 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa 1381 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc 1441 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt 1501 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat 1561 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat 1621 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat 1681 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga 1741 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1801 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1861 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt 1921 atagtcgaat atccctatca gtgatagaga tactagagaa agaggagaaa tactagatgc 1981 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 2041 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 2101 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 2161 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 2221 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 2281 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 2341 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 2401 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 2461 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 2521 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 2581 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 2641 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 2701 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2761 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2821 ttctgcgttt ata //