##gff-version 3
##sequence-region BBa_K176142 1 2833
BBa_K176142	.	promoter	1	35	.	+	0	ID=BBa_J23109;Name=BBa_J23109
BBa_K176142	.	engineered_region	44	2833	.	+	0	ID=BBa_K176134;Name=BBa_K176134
BBa_K176142	.	engineered_region	892	2833	.	+	0	ID=BBa_K176126;Name=BBa_K176126;Parent=BBa_K176134
BBa_K176142	.	engineered_region	935	2833	.	+	0	ID=BBa_K176124;Name=BBa_K176124;Parent=BBa_K176126
BBa_K176142	.	engineered_region	1879	2833	.	+	0	ID=BBa_K176002;Name=BBa_K176002;Parent=BBa_K176124
BBa_K176142	.	ribosome_entry_site	1959	1970	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176002
BBa_K176142	.	conserved_region	1963	1966	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176142	.	promoter	1879	1950	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176002
BBa_K176142	.	non_covalent_binding_site	1932	1950	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176142	.	start_codon	1931	1931	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176142	.	non_covalent_binding_site	1879	1898	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176142	.	promoter	1898	1903	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176142	.	engineered_region	1879	1931	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176142	.	promoter	1920	1925	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176142	.	CDS	1977	2696	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K176002
BBa_K176142	.	CDS	1977	2696	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176142	.	terminator	2705	2784	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176002
BBa_K176142	.	engineered_region	2705	2784	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176142	.	stem_loop	2716	2759	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176142	.	terminator	2793	2833	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176002
BBa_K176142	.	stem_loop	2800	2819	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176142	.	engineered_region	2793	2833	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176142	.	polyA_site	2820	2833	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176142	.	stop_codon	2826	2826	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176142	.	engineered_region	935	1870	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176124
BBa_K176142	.	CDS	953	1708	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176142	.	engineered_region	953	954	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176142	.	engineered_region	953	1708	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176142	.	engineered_tag	1709	1733	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176142	.	CDS	953	1702	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176142	.	point_mutation	1126	1126	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176142	.	point_mutation	1444	1444	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176142	.	terminator	1830	1870	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176142	.	stem_loop	1837	1856	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176142	.	engineered_region	1830	1870	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176142	.	polyA_site	1857	1870	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176142	.	stop_codon	1863	1863	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176142	.	ribosome_entry_site	935	946	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176142	.	conserved_region	939	942	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176142	.	terminator	1742	1821	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176142	.	engineered_region	1742	1821	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176142	.	stem_loop	1753	1796	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176142	.	promoter	892	926	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176126
BBa_K176142	.	promoter	915	920	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176142	.	sequence_alteration	908	908	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176142	.	promoter	892	897	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176142	.	sequence_feature	921	926	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176142	.	sequence_feature	909	914	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176142	.	sequence_feature	898	903	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176142	.	engineered_region	44	883	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176134
BBa_K176142	.	engineered_region	44	746	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176142	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176142	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176142	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176142	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176142	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176142	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176142	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176142	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176142	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176142	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176142	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176142	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176142	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176142	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176142	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K176142
tttacagctagctcagtcctagggactgtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaata
ctagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatatt
aatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatgg
ttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaata
tgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaa
aaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaaca
atggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaa
cataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattta
accaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgca
gtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaa
agcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacag
gtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata