BBa_K176156
type: ComponentDefinition
persistent identity: BBa K176156
display id: BBa_K176156
version: 1
was generated by: iGEM to SBOL conversion
description: Cell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176154 K176063
owned by: james
owned by: myers
mutable description: Cell Death = (Cell Density * pCon 0.01) + Input AHL
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Input: AHL Output1: AHL Output2: Cell Density Design: pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. pCon 0.36(K176009) generates LuxR. pLux/Tet(K176000) generates lacZalpha-ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K176063
component: BBa_K176154
sequence annotation: BBa_K176154
sequence annotation: BBa_K176063
sequence: https://synbiohub.org/public/igem/BBa_K176156_sequence/1

BBa_K176063
type: Component
persistent identity: component2309003
display id: component2309003
version: 1
top level: BBa_K176156
definition: BBa_K176063
access: public

BBa_K176154
type: Component
persistent identity: component2308981
display id: component2308981
version: 1
top level: BBa_K176156
definition: BBa_K176154
access: public

BBa_K176154
type: SequenceAnnotation
persistent identity: annotation2308981
display id: annotation2308981
version: 1
top level: BBa_K176156
location: https://synbiohub.org/public/igem/BBa_K176156/annotation2308981/range2308981/1
component: BBa_K176154

https://synbiohub.org/public/igem/BBa_K176156/annotation2308981/range2308981/1
type: Range
persistent identity: range2308981
display id: range2308981
version: 1
top level: BBa_K176156
direction: direction/reverse
start: 1
end: 1908
orientation: inline

BBa_K176063
type: SequenceAnnotation
persistent identity: annotation2309003
display id: annotation2309003
version: 1
top level: BBa_K176156
location: https://synbiohub.org/public/igem/BBa_K176156/annotation2309003/range2309003/1
component: BBa_K176063

https://synbiohub.org/public/igem/BBa_K176156/annotation2309003/range2309003/1
type: Range
persistent identity: range2309003
display id: range2309003
version: 1
top level: BBa_K176156
direction: direction/reverse
start: 1917
end: 2553
orientation: inline

BBa_K176154
type: ComponentDefinition
persistent identity: BBa K176154
display id: BBa_K176154
version: 1
was generated by: iGEM to SBOL conversion
description: cell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176024 K176125
owned by: james
owned by: myers
mutable description: Output PoPS = (Cell Density * pCon 0.01) + Input AHL
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Input: AHL Output1: AHL Output2: PoPS Design: pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. pCon 0.36(K176009) generates LuxR. pLux/Tet(K176000) generates Output2 PoPS, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K176024
component: BBa_K176125
sequence annotation: BBa_K176024
sequence annotation: BBa_K176125
sequence: https://synbiohub.org/public/igem/BBa_K176154_sequence/1

BBa_K176024
type: Component
persistent identity: component2294288
display id: component2294288
version: 1
top level: BBa_K176154
definition: BBa_K176024
access: public

BBa_K176125
type: Component
persistent identity: component2294319
display id: component2294319
version: 1
top level: BBa_K176154
definition: BBa_K176125
access: public

BBa_K176024
type: SequenceAnnotation
persistent identity: annotation2294288
display id: annotation2294288
version: 1
top level: BBa_K176154
location: https://synbiohub.org/public/igem/BBa_K176154/annotation2294288/range2294288/1
component: BBa_K176024

https://synbiohub.org/public/igem/BBa_K176154/annotation2294288/range2294288/1
type: Range
persistent identity: range2294288
display id: range2294288
version: 1
top level: BBa_K176154
direction: direction/reverse
start: 1
end: 841
orientation: inline

BBa_K176125
type: SequenceAnnotation
persistent identity: annotation2294319
display id: annotation2294319
version: 1
top level: BBa_K176154
location: https://synbiohub.org/public/igem/BBa_K176154/annotation2294319/range2294319/1
component: BBa_K176125

https://synbiohub.org/public/igem/BBa_K176154/annotation2294319/range2294319/1
type: Range
persistent identity: range2294319
display id: range2294319
version: 1
top level: BBa_K176154
direction: direction/reverse
start: 850
end: 1908
orientation: inline

BBa_K176063
type: ComponentDefinition
persistent identity: BBa K176063
display id: BBa_K176063
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->RBS(weak)+lacZalpha-ccdB+T
created: 2009-08-06T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176061 B0015
owned by: james
owned by: myers
mutable description: Generate lacZalpha-ccdB (weak)
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: weak RBS + lacZalpha-ccdB coding + strong terminator
star: false
creator: Zongxiao He, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_K176003
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_K176003
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K176063_sequence/1

BBa_B0012
type: Component
persistent identity: component2015454
display id: component2015454
version: 1
top level: BBa_K176063
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2015452
display id: component2015452
version: 1
top level: BBa_K176063
definition: BBa_B0010
access: public

BBa_K176003
type: Component
persistent identity: component2015451
display id: component2015451
version: 1
top level: BBa_K176063
definition: lacZa-ccdB
access: public

BBa_B0031
type: Component
persistent identity: component2015438
display id: component2015438
version: 1
top level: BBa_K176063
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2015438
display id: annotation2015438
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015438/range2015438/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K176063/annotation2015438/range2015438/1
type: Range
persistent identity: range2015438
display id: range2015438
version: 1
top level: BBa_K176063
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_K176003
type: SequenceAnnotation
persistent identity: annotation2015451
display id: annotation2015451
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015451/range2015451/1
component: BBa_K176003

https://synbiohub.org/public/igem/BBa_K176063/annotation2015451/range2015451/1
type: Range
persistent identity: range2015451
display id: range2015451
version: 1
top level: BBa_K176063
direction: direction/reverse
start: 21
end: 500
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2015452
display id: annotation2015452
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015452/range2015452/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K176063/annotation2015452/range2015452/1
type: Range
persistent identity: range2015452
display id: range2015452
version: 1
top level: BBa_K176063
direction: direction/forward
start: 509
end: 588
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2015454
display id: annotation2015454
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015454/range2015454/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K176063/annotation2015454/range2015454/1
type: Range
persistent identity: range2015454
display id: range2015454
version: 1
top level: BBa_K176063
direction: direction/forward
start: 597
end: 637
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K176024
type: ComponentDefinition
persistent identity: BBa K176024
display id: BBa_K176024
version: 1
was generated by: iGEM to SBOL conversion
description: pCon 0.01(J23103)->RBS+luxI-LVA+T
created: 2009-06-30T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: J23103 K082014
owned by: james
owned by: myers
mutable description: pCon 0.01(J23103)+luxI(K082014)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: pCon 0.01(J23103) generate LuxI
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_J23103
component: BBa_K082014
sequence annotation: BBa_J23103
sequence annotation: BBa_K082014
sequence: https://synbiohub.org/public/igem/BBa_K176024_sequence/1

BBa_J23103
type: Component
persistent identity: component2255277
display id: component2255277
version: 1
top level: BBa_K176024
definition: BBa_J23103
access: public

BBa_K082014
type: Component
persistent identity: component2255292
display id: component2255292
version: 1
top level: BBa_K176024
definition: BBa_K082014
access: public

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2255277
display id: annotation2255277
version: 1
top level: BBa_K176024
location: https://synbiohub.org/public/igem/BBa_K176024/annotation2255277/range2255277/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K176024/annotation2255277/range2255277/1
type: Range
persistent identity: range2255277
display id: range2255277
version: 1
top level: BBa_K176024
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2255292
display id: annotation2255292
version: 1
top level: BBa_K176024
location: https://synbiohub.org/public/igem/BBa_K176024/annotation2255292/range2255292/1
component: BBa_K082014

https://synbiohub.org/public/igem/BBa_K176024/annotation2255292/range2255292/1
type: Range
persistent identity: range2255292
display id: range2255292
version: 1
top level: BBa_K176024
direction: direction/reverse
start: 44
end: 841
orientation: inline

lacZa-ccdB
type: ComponentDefinition
persistent identity: BBa K176003
display id: BBa_K176003
version: 1
was generated by: iGEM to SBOL conversion
description: lacZalpha-ccdB coding sequence
created: 2009-07-02T11:00:00Z
modified: 2015-07-09T11:37:13Z
mutable provenance: PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You). Forward Primer: GTTTCT TCTAG ATG accatgattacgg attcactggccgtcgttttac Reverse Primer: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TTATTA tattccccagaacatcaggtta Digest with XbaI and SpeI, ligate into pSB1A3.
owned by: james
owned by: myers
mutable description: A fusion protein of lacZalpha(I732006) and ccdB(K145151). It should have the function of ccdB to cause E. coli cells to die, but have less toxicity because of the lacZalpha part. It should also have the function of lacZalpha as a reporter for measurement. This part is similar to J32026 but the multiple cloning sites are eliminated. It is constructed by PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You).
discontinued: true
dominant: false
experience: experience/Works
group u list: _282_
m user id: 4206
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: true
mutable notes: The part is similar to BBa_J32026 and the original coding sequence in pluxCcdB3, but the multiple cloning sites are eliminated in order to avoid site-specific mutagenesis. The multiple cloning sites are from lacZalpha in pZErO-2. After eliminate the MCS, the lacZalpha part is identical to the N-terminal of the wild type gene(BBa_I732006 & BBa_I732005), and fused with the ccdB part (BBa_K145151) with a 3aa linker.
star: false
creator: Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacZalpha-ccdB
sequence annotation: Start
sequence annotation: a part of lacZalpha (I732006)
sequence annotation: 177bp and a in J32026
sequence annotation: M13 -20 primer
sequence annotation: M13 -40 primer
sequence annotation: M13 -47 primer (K176059)
sequence annotation: g and 69bp in I732006
sequence annotation: ccdB (K145151)
sequence annotation: at in K145151
sequence annotation: VRccdB primer (K176058)
sequence annotation: c in K145151
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K176003_sequence/1

lacZalpha-ccdB
type: SequenceAnnotation
persistent identity: annotation2020665
display id: annotation2020665
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
type: Range
persistent identity: range2020665
display id: range2020665
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 474
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2008001
display id: annotation2008001
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
type: Range
persistent identity: range2008001
display id: range2008001
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

a part of lacZalpha (I732006)
type: SequenceAnnotation
persistent identity: annotation2008000
display id: annotation2008000
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
type: Range
persistent identity: range2008000
display id: range2008000
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 165
orientation: inline

177bp and a in J32026
type: SequenceAnnotation
persistent identity: annotation2008002
display id: annotation2008002
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
type: Range
persistent identity: range2008002
display id: range2008002
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 16
end: 16
orientation: inline

M13 -20 primer
type: SequenceAnnotation
persistent identity: annotation2008003
display id: annotation2008003
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
type: Range
persistent identity: range2008003
display id: range2008003
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 21
end: 37
orientation: inline

M13 -40 primer
type: SequenceAnnotation
persistent identity: annotation2008005
display id: annotation2008005
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
type: Range
persistent identity: range2008005
display id: range2008005
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 57
orientation: inline

M13 -47 primer (K176059)
type: SequenceAnnotation
persistent identity: annotation2008004
display id: annotation2008004
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
type: Range
persistent identity: range2008004
display id: range2008004
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 64
orientation: inline

g and 69bp in I732006
type: SequenceAnnotation
persistent identity: annotation2008006
display id: annotation2008006
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
type: Range
persistent identity: range2008006
display id: range2008006
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 165
end: 165
orientation: inline

ccdB (K145151)
type: SequenceAnnotation
persistent identity: annotation2008008
display id: annotation2008008
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
type: Range
persistent identity: range2008008
display id: range2008008
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 477
orientation: inline

at in K145151
type: SequenceAnnotation
persistent identity: annotation2008007
display id: annotation2008007
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
type: Range
persistent identity: range2008007
display id: range2008007
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 173
orientation: inline

VRccdB primer (K176058)
type: SequenceAnnotation
persistent identity: annotation2011971
display id: annotation2011971
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
type: Range
persistent identity: range2011971
display id: range2011971
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 188
end: 215
orientation: inline

c in K145151
type: SequenceAnnotation
persistent identity: annotation2008009
display id: annotation2008009
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
type: Range
persistent identity: range2008009
display id: range2008009
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 255
end: 255
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2008010
display id: annotation2008010
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
type: Range
persistent identity: range2008010
display id: range2008010
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 475
end: 480
orientation: inline

BBa_K176125
type: ComponentDefinition
persistent identity: BBa K176125
display id: BBa_K176125
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPS
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: K176009 K176123
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible High LuxR level Similar to F2620, but use pLux/Tet hybrid promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Input: AHL Output: PoPS Design: pCon 0.36(K176009) generates LuxR pLux/Tet(K176000) generates Output PoPS, activated by LuxR and Input AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_K176123
component: BBa_K176009
sequence annotation: BBa_K176009
sequence annotation: BBa_K176123
sequence: https://synbiohub.org/public/igem/BBa_K176125_sequence/1

BBa_K176123
type: Component
persistent identity: component2262819
display id: component2262819
version: 1
top level: BBa_K176125
definition: BBa_K176123
access: public

BBa_K176009
type: Component
persistent identity: component2262796
display id: component2262796
version: 1
top level: BBa_K176125
definition: pCon 0.36
access: public

BBa_K176009
type: SequenceAnnotation
persistent identity: annotation2262796
display id: annotation2262796
version: 1
top level: BBa_K176125
location: https://synbiohub.org/public/igem/BBa_K176125/annotation2262796/range2262796/1
component: BBa_K176009

https://synbiohub.org/public/igem/BBa_K176125/annotation2262796/range2262796/1
type: Range
persistent identity: range2262796
display id: range2262796
version: 1
top level: BBa_K176125
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K176123
type: SequenceAnnotation
persistent identity: annotation2262819
display id: annotation2262819
version: 1
top level: BBa_K176125
location: https://synbiohub.org/public/igem/BBa_K176125/annotation2262819/range2262819/1
component: BBa_K176123

https://synbiohub.org/public/igem/BBa_K176125/annotation2262819/range2262819/1
type: Range
persistent identity: range2262819
display id: range2262819
version: 1
top level: BBa_K176125
direction: direction/reverse
start: 44
end: 1059
orientation: inline

BBa_K082014
type: ComponentDefinition
persistent identity: BBa K082014
display id: BBa_K082014
version: 1
was generated by: iGEM to SBOL conversion
description: C0261-B0015
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: none
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: none
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Zhen Xu , Binjie Xu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0261
sequence annotation: BBa_C0261
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K082014_sequence/1

BBa_B0015
type: Component
persistent identity: component2249441
display id: component2249441
version: 1
top level: BBa_K082014
definition: BBa_B0015
access: public

BBa_C0261
type: Component
persistent identity: component2249434
display id: component2249434
version: 1
top level: BBa_K082014
definition: BBa_C0261
access: public

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2249434
display id: annotation2249434
version: 1
top level: BBa_K082014
location: https://synbiohub.org/public/igem/BBa_K082014/annotation2249434/range2249434/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K082014/annotation2249434/range2249434/1
type: Range
persistent identity: range2249434
display id: range2249434
version: 1
top level: BBa_K082014
direction: direction/reverse
start: 1
end: 661
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249441
display id: annotation2249441
version: 1
top level: BBa_K082014
location: https://synbiohub.org/public/igem/BBa_K082014/annotation2249441/range2249441/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082014/annotation2249441/range2249441/1
type: Range
persistent identity: range2249441
display id: range2249441
version: 1
top level: BBa_K082014
direction: direction/reverse
start: 670
end: 798
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

pCon 0.36
type: ComponentDefinition
persistent identity: BBa K176009
display id: BBa_K176009
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member J23107 actual sequence (pCon 0.36)
created: 2009-06-19T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: This is the actual sequence of J23107.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The sequence of J23107 is wrong according to sequencing result of the registry and ours. This part K176009 is created for the actual promoter. http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=3628 http://partsregistry.org/cgi/sequencing/one_blast.cgi?id=1184 J23107: tttacggctagctcagCcctaggtattatgctagc K176009: tttacggctagctcagTcctaggtattatgctagc
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: J23107
star: false
creator: Hao Jiang, Danqian Liu, Chao Li
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: NheI
sequence annotation: c in J23107
sequence annotation: AvrII
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K176009_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2005855
display id: annotation2005855
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005855/range2005855/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K176009/annotation2005855/range2005855/1
type: Range
persistent identity: range2005855
display id: range2005855
version: 1
top level: pCon 0.36
direction: direction/forward
start: 1
end: 6
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2005856
display id: annotation2005856
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005856/range2005856/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005856/range2005856/1
type: Range
persistent identity: range2005856
display id: range2005856
version: 1
top level: pCon 0.36
direction: direction/forward
start: 7
end: 12
orientation: inline

c in J23107
type: SequenceAnnotation
persistent identity: annotation2005857
display id: annotation2005857
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005857/range2005857/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K176009/annotation2005857/range2005857/1
type: Range
persistent identity: range2005857
display id: range2005857
version: 1
top level: pCon 0.36
direction: direction/forward
start: 17
end: 17
orientation: inline

AvrII
type: SequenceAnnotation
persistent identity: annotation2005858
display id: annotation2005858
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005858/range2005858/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005858/range2005858/1
type: Range
persistent identity: range2005858
display id: range2005858
version: 1
top level: pCon 0.36
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2005859
display id: annotation2005859
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005859/range2005859/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K176009/annotation2005859/range2005859/1
type: Range
persistent identity: range2005859
display id: range2005859
version: 1
top level: pCon 0.36
direction: direction/forward
start: 24
end: 29
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2005860
display id: annotation2005860
version: 1
top level: pCon 0.36
location: https://synbiohub.org/public/igem/BBa_K176009/annotation2005860/range2005860/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K176009/annotation2005860/range2005860/1
type: Range
persistent identity: range2005860
display id: range2005860
version: 1
top level: pCon 0.36
direction: direction/forward
start: 30
end: 35
orientation: inline

BBa_K176123
type: ComponentDefinition
persistent identity: BBa K176123
display id: BBa_K176123
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (PoPS->luxR): PoPS->luxR+pLux/Tet->PoPS
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:03Z
mutable provenance: I0462 K176000
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible Similar to F2620, but use pLux/Tet hybrid promoter. Different input PoPS result different luxR level, K176028, K176125, K176127, K176129.
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Input1: PoPS Input2: AHL Output: PoPS Design: Input1 PoPS generates LuxR pLux/Tet(K176000) generates PoPS output, activated by LuxR and Input2 AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_I0462
component: BBa_K176000
sequence annotation: BBa_I0462
sequence annotation: BBa_K176000
sequence: https://synbiohub.org/public/igem/BBa_K176123_sequence/1

BBa_I0462
type: Component
persistent identity: component2255854
display id: component2255854
version: 1
top level: BBa_K176123
definition: LuxR
access: public

BBa_K176000
type: Component
persistent identity: component2255861
display id: component2255861
version: 1
top level: BBa_K176123
definition: pLux/Tet
access: public

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2255854
display id: annotation2255854
version: 1
top level: BBa_K176123
location: https://synbiohub.org/public/igem/BBa_K176123/annotation2255854/range2255854/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K176123/annotation2255854/range2255854/1
type: Range
persistent identity: range2255854
display id: range2255854
version: 1
top level: BBa_K176123
direction: direction/reverse
start: 1
end: 936
orientation: inline

BBa_K176000
type: SequenceAnnotation
persistent identity: annotation2255861
display id: annotation2255861
version: 1
top level: BBa_K176123
location: https://synbiohub.org/public/igem/BBa_K176123/annotation2255861/range2255861/1
component: BBa_K176000

https://synbiohub.org/public/igem/BBa_K176123/annotation2255861/range2255861/1
type: Range
persistent identity: range2255861
display id: range2255861
version: 1
top level: BBa_K176123
direction: direction/reverse
start: 945
end: 1016
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-making Enzyme, luxI (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0034.C0061
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
top level: BBa_C0261
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
top level: BBa_C0261
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K176156_sequence/1
type: Sequence
persistent identity: BBa K176156 sequence
display id: BBa_K176156_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176154_sequence/1
type: Sequence
persistent identity: BBa K176154 sequence
display id: BBa_K176154_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176063_sequence/1
type: Sequence
persistent identity: BBa K176063 sequence
display id: BBa_K176063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176024_sequence/1
type: Sequence
persistent identity: BBa K176024 sequence
display id: BBa_K176024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176003_sequence/1
type: Sequence
persistent identity: BBa K176003 sequence
display id: BBa_K176003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176125_sequence/1
type: Sequence
persistent identity: BBa K176125 sequence
display id: BBa_K176125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082014_sequence/1
type: Sequence
persistent identity: BBa K082014 sequence
display id: BBa_K082014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23103_sequence/1
type: Sequence
persistent identity: BBa J23103 sequence
display id: BBa_J23103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176009_sequence/1
type: Sequence
persistent identity: BBa K176009 sequence
display id: BBa_K176009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176123_sequence/1
type: Sequence
persistent identity: BBa K176123 sequence
display id: BBa_K176123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0261_sequence/1
type: Sequence
persistent identity: BBa C0261 sequence
display id: BBa_C0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z