LOCUS       BBa_K176158              513 bp    DNA     linear       01-May-2024
DEFINITION  lacZalpha-ccdB-LVA coding sequence
ACCESSION   BBa_K176158
VERSION     BBa_K176158.1  
FEATURES             Location/Qualifiers
     CDS             1..474
                     /label=lacZalpha-ccdB
     misc_feature    475..507
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    508..513
                     /label=Stop
     misc_feature    1..165
                     /label=a part of lacZalpha (I732006)
     misc_feature    1..3
                     /label=Start
     misc_feature    16..16
                     /label=177bp and a in J32026
     primer_bind     21..37
                     /label=M13 -20 primer
     primer_bind     41..64
                     /label=M13 -47 primer (K176059)
     primer_bind     41..57
                     /label=M13 -40 primer
     misc_feature    165..165
                     /label=g and 69bp in I732006
     misc_feature    172..173
                     /label=at in K145151
     misc_feature    172..474
                     /label=ccdB (K145151)
     primer_bind     188..215
                     /label=VRccdB primer (K176058)
     misc_feature    255..255
                     /label=c in K145151
ORIGIN
        1 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt acggcagttt
      181 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
      241 attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
      301 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
      361 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
      421 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatagctgca
      481 aacgacgaaa actacgcttt agtagcttaa taa
//