##gff-version 3
##sequence-region BBa_K176158 1 513
BBa_K176158	.	primer_binding_site	41	57	.	+	0	ID=annotation2020922;Name=M13 -40 primer
BBa_K176158	.	sequence_alteration	172	173	.	+	0	ID=annotation2020924;Name=at in K145151
BBa_K176158	.	point_mutation	165	165	.	+	0	ID=annotation2020923;Name=g and 69bp in I732006
BBa_K176158	.	point_mutation	255	255	.	+	0	ID=annotation2020927;Name=c in K145151
BBa_K176158	.	start_codon	1	3	.	+	0	ID=annotation2020918;Name=Start
BBa_K176158	.	stop_codon	508	513	.	+	0	ID=annotation2020916;Name=Stop
BBa_K176158	.	CDS	1	474	.	+	0	ID=annotation2020913;Name=lacZalpha-ccdB
BBa_K176158	.	sequence_alteration	16	16	.	+	0	ID=annotation2020919;Name=177bp and a in J32026
BBa_K176158	.	engineered_region	1	165	.	+	0	ID=annotation2020917;Name=a part of lacZalpha (I732006)
BBa_K176158	.	engineered_region	172	474	.	+	0	ID=annotation2020925;Name=ccdB (K145151)
BBa_K176158	.	primer_binding_site	41	64	.	+	0	ID=annotation2020921;Name=M13 -47 primer (K176059)
BBa_K176158	.	primer_binding_site	21	37	.	+	0	ID=annotation2020920;Name=M13 -20 primer
BBa_K176158	.	primer_binding_site	188	215	.	+	0	ID=annotation2020926;Name=VRccdB primer (K176058)
BBa_K176158	.	tag	475	507	.	+	0	ID=annotation2020914;Name=LVA degradation tag (AANDENYALVA)
>BBa_K176158
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatc
gccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcg
cagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagt
gatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtg
aactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgt
tatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaata
gctgcaaacgacgaaaactacgctttagtagcttaataa