BBa_K176158 1 lacZa-ccdB lacZalpha-ccdB-LVA coding sequence 2009-09-13T11:00:00Z 2015-07-09T11:38:42Z PCR from K176003. Forward Primer: VF2(G00100) Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta Digest with EcoRI and SpeI, ligate into pSB1A3. Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003) C-terminal: [K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] A A N D E N Y A L V A true false _282_ 4206 3908 9 Not in stock true Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003 C-terminal: [K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] A A N D E N Y A L V A false Danqian Liu, Zongxiao He, Hao Jiang annotation2020924 1 at in K145151 range2020924 1 172 173 annotation2020923 1 g and 69bp in I732006 range2020923 1 165 165 annotation2020914 1 LVA degradation tag (AANDENYALVA) range2020914 1 475 507 annotation2020927 1 c in K145151 range2020927 1 255 255 annotation2020922 1 M13 -40 primer range2020922 1 41 57 annotation2020913 1 lacZalpha-ccdB range2020913 1 1 474 annotation2020921 1 M13 -47 primer (K176059) range2020921 1 41 64 annotation2020916 1 Stop range2020916 1 508 513 annotation2020918 1 Start range2020918 1 1 3 annotation2020919 1 177bp and a in J32026 range2020919 1 16 16 annotation2020917 1 a part of lacZalpha (I732006) range2020917 1 1 165 annotation2020925 1 ccdB (K145151) range2020925 1 172 474 annotation2020926 1 VRccdB primer (K176058) range2020926 1 188 215 annotation2020920 1 M13 -20 primer range2020920 1 21 37 BBa_K176158_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z