LOCUS       BBa_K176160              531 bp    DNA     linear       27-Jun-2024
DEFINITION  PoPS->RBS+lacZalpha-ccdB-LVA->PoPS
ACCESSION   BBa_K176160
VERSION     BBa_K176160.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..492
                     /label=lacZalpha-ccdB
     misc_feature    493..525
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    526..531
                     /label=Stop
     misc_feature    19..183
                     /label=a part of lacZalpha (I732006)
     misc_feature    19..21
                     /label=Start
     misc_feature    34..34
                     /label=177bp and a in J32026
     primer_bind     39..55
                     /label=M13 -20 primer
     primer_bind     59..82
                     /label=M13 -47 primer (K176059)
     primer_bind     59..75
                     /label=M13 -40 primer
     misc_feature    183..183
                     /label=g and 69bp in I732006
     misc_feature    190..191
                     /label=at in K145151
     misc_feature    190..492
                     /label=ccdB (K145151)
     primer_bind     206..233
                     /label=VRccdB primer (K176058)
     misc_feature    273..273
                     /label=c in K145151
     CDS             19..531
                     /label=BBa_K176158
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
      241 gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc
      301 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
      361 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
      421 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
      481 ttctggggaa tagctgcaaa cgacgaaaac tacgctttag tagcttaata a
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