##gff-version 3
##sequence-region BBa_K176160 1 531
BBa_K176160	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K176160	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176160	.	CDS	19	531	.	+	0	ID=BBa_K176158;Name=BBa_K176158
BBa_K176160	.	CDS	19	492	.	+	0	ID=annotation2020913;Name=lacZalpha-ccdB;Parent=BBa_K176158
BBa_K176160	.	primer_binding_site	59	75	.	+	0	ID=annotation2020922;Name=M13 -40 primer;Parent=BBa_K176158
BBa_K176160	.	start_codon	19	21	.	+	0	ID=annotation2020918;Name=Start;Parent=BBa_K176158
BBa_K176160	.	primer_binding_site	59	82	.	+	0	ID=annotation2020921;Name=M13 -47 primer (K176059);Parent=BBa_K176158
BBa_K176160	.	primer_binding_site	39	55	.	+	0	ID=annotation2020920;Name=M13 -20 primer;Parent=BBa_K176158
BBa_K176160	.	engineered_region	19	183	.	+	0	ID=annotation2020917;Name=a part of lacZalpha (I732006);Parent=BBa_K176158
BBa_K176160	.	engineered_region	190	492	.	+	0	ID=annotation2020925;Name=ccdB (K145151);Parent=BBa_K176158
BBa_K176160	.	sequence_alteration	34	34	.	+	0	ID=annotation2020919;Name=177bp and a in J32026;Parent=BBa_K176158
BBa_K176160	.	stop_codon	526	531	.	+	0	ID=annotation2020916;Name=Stop;Parent=BBa_K176158
BBa_K176160	.	point_mutation	273	273	.	+	0	ID=annotation2020927;Name=c in K145151;Parent=BBa_K176158
BBa_K176160	.	primer_binding_site	206	233	.	+	0	ID=annotation2020926;Name=VRccdB primer (K176058);Parent=BBa_K176158
BBa_K176160	.	point_mutation	183	183	.	+	0	ID=annotation2020923;Name=g and 69bp in I732006;Parent=BBa_K176158
BBa_K176160	.	tag	493	525	.	+	0	ID=annotation2020914;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176158
BBa_K176160	.	sequence_alteration	190	191	.	+	0	ID=annotation2020924;Name=at in K145151;Parent=BBa_K176158
>BBa_K176160
aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctg
gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg
cccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctg
tttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgt
cagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggc
cagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaac
ctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa