BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_K176161
1
BBa_K176161
PoPS->RBS(weak)+ccdB-LVA->PoPS
2009-09-21T11:00:00Z
2015-05-08T01:11:03Z
PCR from K176060.
Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
RBS(weak) + ccdB-LVA
false
false
_282_
0
3908
9
Not in stock
false
weak RBS + ccdB-LVA coding
false
Danqian Liu, Zongxiao He, Hao Jiang
component2026635
1
BBa_K176157
component2026629
1
BBa_B0031
annotation2026635
1
BBa_K176157
range2026635
1
21
362
annotation2026629
1
BBa_B0031
range2026629
1
1
14
BBa_K176157
1
ccdB-LVA
ccdB-LVA coding sequence
2009-09-13T11:00:00Z
2015-07-09T11:35:55Z
PCR from K145151.
Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:
[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
true
false
_282_
4206
3908
9
It's complicated
true
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:
[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
false
Danqian Liu, Zongxiao He, Hao Jiang
annotation2020910
1
Start
range2020910
1
1
3
annotation2020909
1
LVA degradation tag (AANDENYALVA)
range2020909
1
304
336
annotation2020911
1
Stop
range2020911
1
337
342
annotation2020908
1
ccdB (K145151)
range2020908
1
1
303
annotation2020912
1
VRccdB primer (K176058)
range2020912
1
17
44
BBa_K176161_sequence
1
tcacacaggaaacctactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_K176157_sequence
1
atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_B0031_sequence
1
tcacacaggaaacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z