BBa_K176158 1 lacZa-ccdB lacZalpha-ccdB-LVA coding sequence 2009-09-13T11:00:00Z 2015-07-09T11:38:42Z PCR from K176003. Forward Primer: VF2(G00100) Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta Digest with EcoRI and SpeI, ligate into pSB1A3. Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003) C-terminal: [K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] A A N D E N Y A L V A true false _282_ 4206 3908 9 Not in stock true Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003 C-terminal: [K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] A A N D E N Y A L V A false Danqian Liu, Zongxiao He, Hao Jiang annotation2020920 1 M13 -20 primer range2020920 1 21 37 annotation2020927 1 c in K145151 range2020927 1 255 255 annotation2020914 1 LVA degradation tag (AANDENYALVA) range2020914 1 475 507 annotation2020919 1 177bp and a in J32026 range2020919 1 16 16 annotation2020923 1 g and 69bp in I732006 range2020923 1 165 165 annotation2020916 1 Stop range2020916 1 508 513 annotation2020925 1 ccdB (K145151) range2020925 1 172 474 annotation2020921 1 M13 -47 primer (K176059) range2020921 1 41 64 annotation2020922 1 M13 -40 primer range2020922 1 41 57 annotation2020926 1 VRccdB primer (K176058) range2020926 1 188 215 annotation2020917 1 a part of lacZalpha (I732006) range2020917 1 1 165 annotation2020913 1 lacZalpha-ccdB range2020913 1 1 474 annotation2020918 1 Start range2020918 1 1 3 annotation2020924 1 at in K145151 range2020924 1 172 173 BBa_B0031 1 BBa_B0031 RBS.2 (weak) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false <P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-1&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23316 1 conserved range23316 1 7 10 BBa_K176162 1 BBa_K176162 PoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPS 2009-09-21T11:00:00Z 2015-05-08T01:11:03Z PCR from K176061. Forward Primer: VF2(G00100) Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta Digest with EcoRI and SpeI, ligate into pSB1A3. RBS(weak) + lacZalpha-ccdB-LVA false false _282_ 0 3908 9 It's complicated false weak RBS + lacZalpha-ccdB-LVA coding false Danqian Liu, Zongxiao He, Hao Jiang component2026612 1 BBa_B0031 component2026627 1 BBa_K176158 annotation2026612 1 BBa_B0031 range2026612 1 1 14 annotation2026627 1 BBa_K176158 range2026627 1 21 533 BBa_K176158_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa BBa_K176162_sequence 1 tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa BBa_B0031_sequence 1 tcacacaggaaacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z