BBa_K176158
1
lacZa-ccdB
lacZalpha-ccdB-LVA coding sequence
2009-09-13T11:00:00Z
2015-07-09T11:38:42Z
PCR from K176003.
Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003) C-terminal:
[K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
true
false
_282_
4206
3908
9
Not in stock
true
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003 C-terminal:
[K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
false
Danqian Liu, Zongxiao He, Hao Jiang
annotation2020920
1
M13 -20 primer
range2020920
1
21
37
annotation2020927
1
c in K145151
range2020927
1
255
255
annotation2020914
1
LVA degradation tag (AANDENYALVA)
range2020914
1
475
507
annotation2020919
1
177bp and a in J32026
range2020919
1
16
16
annotation2020923
1
g and 69bp in I732006
range2020923
1
165
165
annotation2020916
1
Stop
range2020916
1
508
513
annotation2020925
1
ccdB (K145151)
range2020925
1
172
474
annotation2020921
1
M13 -47 primer (K176059)
range2020921
1
41
64
annotation2020922
1
M13 -40 primer
range2020922
1
41
57
annotation2020926
1
VRccdB primer (K176058)
range2020926
1
188
215
annotation2020917
1
a part of lacZalpha (I732006)
range2020917
1
1
165
annotation2020913
1
lacZalpha-ccdB
range2020913
1
1
474
annotation2020918
1
Start
range2020918
1
1
3
annotation2020924
1
at in K145151
range2020924
1
172
173
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_K176162
1
BBa_K176162
PoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPS
2009-09-21T11:00:00Z
2015-05-08T01:11:03Z
PCR from K176061.
Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
RBS(weak) + lacZalpha-ccdB-LVA
false
false
_282_
0
3908
9
It's complicated
false
weak RBS + lacZalpha-ccdB-LVA coding
false
Danqian Liu, Zongxiao He, Hao Jiang
component2026612
1
BBa_B0031
component2026627
1
BBa_K176158
annotation2026612
1
BBa_B0031
range2026612
1
1
14
annotation2026627
1
BBa_K176158
range2026627
1
21
533
BBa_K176158_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_K176162_sequence
1
tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_B0031_sequence
1
tcacacaggaaacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z