LOCUS       BBa_K176163              497 bp    DNA     linear       16-Jun-2024
DEFINITION  PoPS->RBS+ccdB-LVA+T
ACCESSION   BBa_K176163
VERSION     BBa_K176163.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..321
                     /label=ccdB (K145151)
     misc_feature    322..354
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    19..21
                     /label=Start
     misc_feature    355..360
                     /label=Stop
     primer_bind     35..62
                     /label=VRccdB primer (K176058)
     CDS             19..360
                     /label=BBa_K176157
     stem_loop       380..423
                     /label=stem_loop
     misc_feature    369..448
                     /label=BBa_B0010
     terminator      369..448
                     /label=BBa_B0010
     stem_loop       464..483
                     /label=T7 TE
     misc_feature    490..490
                     /label=stop
     polyA_site      484..497
                     /label=polya
     misc_feature    457..497
                     /label=BBa_B0012
     terminator      457..497
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcagtttaag gtttacacct ataaaagaga gagccgttat
       61 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
      121 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
      181 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
      241 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
      301 aacctgatgt tctggggaat agctgcaaac gacgaaaact acgctttagt agcttaataa
      361 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      421 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      481 gcctttctgc gtttata
//