##gff-version 3
##sequence-region BBa_K176163 1 497
BBa_K176163	.	terminator	369	448	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K176163	.	engineered_region	369	448	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176163	.	stem_loop	380	423	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176163	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K176163	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176163	.	terminator	457	497	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K176163	.	engineered_region	457	497	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176163	.	stem_loop	464	483	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176163	.	polyA_site	484	497	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176163	.	stop_codon	490	490	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176163	.	CDS	19	360	.	+	0	ID=BBa_K176157;Name=BBa_K176157
BBa_K176163	.	primer_binding_site	35	62	.	+	0	ID=annotation2020912;Name=VRccdB primer (K176058);Parent=BBa_K176157
BBa_K176163	.	tag	322	354	.	+	0	ID=annotation2020909;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176157
BBa_K176163	.	start_codon	19	21	.	+	0	ID=annotation2020910;Name=Start;Parent=BBa_K176157
BBa_K176163	.	CDS	19	321	.	+	0	ID=annotation2020908;Name=ccdB (K145151);Parent=BBa_K176157
BBa_K176163	.	stop_codon	355	360	.	+	0	ID=annotation2020911;Name=Stop;Parent=BBa_K176157
>BBa_K176163
aaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtac
agagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctc
ccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtc
tccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggg
gaatagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata