LOCUS       BBa_K176165              499 bp    DNA     linear       01-Jun-2024
DEFINITION  PoPS->RBS(weak)+ccdB-LVA+T
ACCESSION   BBa_K176165
VERSION     BBa_K176165.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             21..323
                     /label=ccdB (K145151)
     misc_feature    324..356
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    21..23
                     /label=Start
     misc_feature    357..362
                     /label=Stop
     primer_bind     37..64
                     /label=VRccdB primer (K176058)
     CDS             21..362
                     /label=BBa_K176157
     stem_loop       382..425
                     /label=stem_loop
     misc_feature    371..450
                     /label=BBa_B0010
     terminator      371..450
                     /label=BBa_B0010
     stem_loop       466..485
                     /label=T7 TE
     misc_feature    492..492
                     /label=stop
     polyA_site      486..499
                     /label=polya
     misc_feature    459..499
                     /label=BBa_B0012
     terminator      459..499
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aacctactag atgcagttta aggtttacac ctataaaaga gagagccgtt
       61 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
      121 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
      181 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
      241 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
      301 ttaacctgat gttctgggga atagctgcaa acgacgaaaa ctacgcttta gtagcttaat
      361 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      421 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      481 gggcctttct gcgtttata
//