##gff-version 3
##sequence-region BBa_K176165 1 499
BBa_K176165	.	terminator	371	450	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K176165	.	engineered_region	371	450	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176165	.	stem_loop	382	425	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176165	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K176165	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176165	.	terminator	459	499	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K176165	.	stop_codon	492	492	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176165	.	polyA_site	486	499	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176165	.	stem_loop	466	485	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176165	.	engineered_region	459	499	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176165	.	CDS	21	362	.	+	0	ID=BBa_K176157;Name=BBa_K176157
BBa_K176165	.	start_codon	21	23	.	+	0	ID=annotation2020910;Name=Start;Parent=BBa_K176157
BBa_K176165	.	primer_binding_site	37	64	.	+	0	ID=annotation2020912;Name=VRccdB primer (K176058);Parent=BBa_K176157
BBa_K176165	.	CDS	21	323	.	+	0	ID=annotation2020908;Name=ccdB (K145151);Parent=BBa_K176157
BBa_K176165	.	tag	324	356	.	+	0	ID=annotation2020909;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176157
BBa_K176165	.	stop_codon	357	362	.	+	0	ID=annotation2020911;Name=Stop;Parent=BBa_K176157
>BBa_K176165
tcacacaggaaacctactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgt
acagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtc
tcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccgg
tctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctg
gggaatagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata