LOCUS       BBa_K176166              670 bp    DNA     linear       22-Nov-2024
DEFINITION  PoPS->RBS(weak)+lacZalpha-ccdB-LVA+T
ACCESSION   BBa_K176166
VERSION     BBa_K176166.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             21..494
                     /label=lacZalpha-ccdB
     misc_feature    495..527
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    528..533
                     /label=Stop
     misc_feature    21..185
                     /label=a part of lacZalpha (I732006)
     misc_feature    21..23
                     /label=Start
     misc_feature    36..36
                     /label=177bp and a in J32026
     primer_bind     41..57
                     /label=M13 -20 primer
     primer_bind     61..84
                     /label=M13 -47 primer (K176059)
     primer_bind     61..77
                     /label=M13 -40 primer
     misc_feature    185..185
                     /label=g and 69bp in I732006
     misc_feature    192..193
                     /label=at in K145151
     misc_feature    192..494
                     /label=ccdB (K145151)
     primer_bind     208..235
                     /label=VRccdB primer (K176058)
     misc_feature    275..275
                     /label=c in K145151
     CDS             21..533
                     /label=BBa_K176158
     stem_loop       553..596
                     /label=stem_loop
     misc_feature    542..621
                     /label=BBa_B0010
     terminator      542..621
                     /label=BBa_B0010
     stem_loop       637..656
                     /label=T7 TE
     misc_feature    663..663
                     /label=stop
     polyA_site      657..670
                     /label=polya
     misc_feature    630..670
                     /label=BBa_B0012
     terminator      630..670
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aacctactag atgaccatga ttacggattc actggccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      241 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
      301 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
      361 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
      421 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
      481 tgttctgggg aatagctgca aacgacgaaa actacgcttt agtagcttaa taatactaga
      541 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      601 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      661 tgcgtttata
//