LOCUS       BBa_K176167             1564 bp    DNA     linear       22-Nov-2024
DEFINITION  TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccd
            B-LVA
ACCESSION   BBa_K176167
VERSION     BBa_K176167.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_K176025
     misc_feature    988..1040
                     /label=R0062
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1041..1059
                     /label=tetR
     misc_feature    1040..1040
                     /label=+1
     misc_feature    988..1007
                     /label=luxR/HSL
     promoter        988..1059
                     /label=BBa_K176000
     misc_feature    1..1059
                     /label=BBa_K176028
     misc_feature    1072..1075
                     /label=conserved
     RBS             1068..1079
                     /label=BBa_B0034
     CDS             1086..1388
                     /label=ccdB (K145151)
     misc_feature    1389..1421
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    1086..1088
                     /label=Start
     misc_feature    1422..1427
                     /label=Stop
     primer_bind     1102..1129
                     /label=VRccdB primer (K176058)
     CDS             1086..1427
                     /label=BBa_K176157
     stem_loop       1447..1490
                     /label=stem_loop
     misc_feature    1436..1515
                     /label=BBa_B0010
     terminator      1436..1515
                     /label=BBa_B0010
     stem_loop       1531..1550
                     /label=T7 TE
     misc_feature    1557..1557
                     /label=stop
     polyA_site      1551..1564
                     /label=polya
     misc_feature    1524..1564
                     /label=BBa_B0012
     terminator      1524..1564
                     /label=BBa_B0012
     misc_feature    1068..1564
                     /label=BBa_K176163
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat
     1081 actagatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1141 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     1201 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     1261 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     1321 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     1381 ggggaatagc tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg
     1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1561 tata
//