LOCUS       BBa_K176170             1737 bp    DNA     linear       26-Nov-2024
DEFINITION  TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lac
            Zalpha-ccdB-LVA(weak RBS)
ACCESSION   BBa_K176170
VERSION     BBa_K176170.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_K176025
     misc_feature    988..1040
                     /label=R0062
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1041..1059
                     /label=tetR
     misc_feature    1040..1040
                     /label=+1
     misc_feature    988..1007
                     /label=luxR/HSL
     promoter        988..1059
                     /label=BBa_K176000
     misc_feature    1..1059
                     /label=BBa_K176028
     misc_feature    1074..1077
                     /label=conserved
     RBS             1068..1081
                     /label=BBa_B0031
     CDS             1088..1561
                     /label=lacZalpha-ccdB
     misc_feature    1562..1594
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    1595..1600
                     /label=Stop
     misc_feature    1088..1252
                     /label=a part of lacZalpha (I732006)
     misc_feature    1088..1090
                     /label=Start
     misc_feature    1103..1103
                     /label=177bp and a in J32026
     primer_bind     1108..1124
                     /label=M13 -20 primer
     primer_bind     1128..1151
                     /label=M13 -47 primer (K176059)
     primer_bind     1128..1144
                     /label=M13 -40 primer
     misc_feature    1252..1252
                     /label=g and 69bp in I732006
     misc_feature    1259..1260
                     /label=at in K145151
     misc_feature    1259..1561
                     /label=ccdB (K145151)
     primer_bind     1275..1302
                     /label=VRccdB primer (K176058)
     misc_feature    1342..1342
                     /label=c in K145151
     CDS             1088..1600
                     /label=BBa_K176158
     stem_loop       1620..1663
                     /label=stem_loop
     misc_feature    1609..1688
                     /label=BBa_B0010
     terminator      1609..1688
                     /label=BBa_B0010
     stem_loop       1704..1723
                     /label=T7 TE
     misc_feature    1730..1730
                     /label=stop
     polyA_site      1724..1737
                     /label=polya
     misc_feature    1697..1737
                     /label=BBa_B0012
     terminator      1697..1737
                     /label=BBa_B0012
     misc_feature    1068..1737
                     /label=BBa_K176166
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagtca cacaggaaac
     1081 ctactagatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
     1141 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1201 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg
     1261 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     1321 gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca gtgcacgtct
     1381 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     1441 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     1501 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     1561 agctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa
     1621 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1681 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//