##gff-version 3
##sequence-region BBa_K176170 1 1737
BBa_K176170	.	engineered_region	1068	1737	.	+	0	ID=BBa_K176166;Name=BBa_K176166
BBa_K176170	.	terminator	1697	1737	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176166
BBa_K176170	.	polyA_site	1724	1737	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176170	.	engineered_region	1697	1737	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176170	.	stem_loop	1704	1723	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176170	.	stop_codon	1730	1730	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176170	.	CDS	1088	1600	.	+	0	ID=BBa_K176158;Name=BBa_K176158;Parent=BBa_K176166
BBa_K176170	.	sequence_alteration	1259	1260	.	+	0	ID=annotation2020924;Name=at in K145151;Parent=BBa_K176158
BBa_K176170	.	primer_binding_site	1108	1124	.	+	0	ID=annotation2020920;Name=M13 -20 primer;Parent=BBa_K176158
BBa_K176170	.	point_mutation	1342	1342	.	+	0	ID=annotation2020927;Name=c in K145151;Parent=BBa_K176158
BBa_K176170	.	start_codon	1088	1090	.	+	0	ID=annotation2020918;Name=Start;Parent=BBa_K176158
BBa_K176170	.	engineered_region	1088	1252	.	+	0	ID=annotation2020917;Name=a part of lacZalpha (I732006);Parent=BBa_K176158
BBa_K176170	.	CDS	1088	1561	.	+	0	ID=annotation2020913;Name=lacZalpha-ccdB;Parent=BBa_K176158
BBa_K176170	.	tag	1562	1594	.	+	0	ID=annotation2020914;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176158
BBa_K176170	.	sequence_alteration	1103	1103	.	+	0	ID=annotation2020919;Name=177bp and a in J32026;Parent=BBa_K176158
BBa_K176170	.	engineered_region	1259	1561	.	+	0	ID=annotation2020925;Name=ccdB (K145151);Parent=BBa_K176158
BBa_K176170	.	stop_codon	1595	1600	.	+	0	ID=annotation2020916;Name=Stop;Parent=BBa_K176158
BBa_K176170	.	primer_binding_site	1128	1151	.	+	0	ID=annotation2020921;Name=M13 -47 primer (K176059);Parent=BBa_K176158
BBa_K176170	.	primer_binding_site	1128	1144	.	+	0	ID=annotation2020922;Name=M13 -40 primer;Parent=BBa_K176158
BBa_K176170	.	primer_binding_site	1275	1302	.	+	0	ID=annotation2020926;Name=VRccdB primer (K176058);Parent=BBa_K176158
BBa_K176170	.	point_mutation	1252	1252	.	+	0	ID=annotation2020923;Name=g and 69bp in I732006;Parent=BBa_K176158
BBa_K176170	.	ribosome_entry_site	1068	1081	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176166
BBa_K176170	.	conserved_region	1074	1077	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176170	.	terminator	1609	1688	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176166
BBa_K176170	.	stem_loop	1620	1663	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176170	.	engineered_region	1609	1688	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176170	.	engineered_region	1	1059	.	+	0	ID=BBa_K176028;Name=BBa_K176028
BBa_K176170	.	engineered_region	1	979	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176170	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176170	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176170	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176170	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176170	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176170	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176170	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176170	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176170	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176170	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176170	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176170	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176170	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176170	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176170	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176170	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176170	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176170	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176170	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176170	.	promoter	988	1059	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176170	.	promoter	1029	1034	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176170	.	promoter	1007	1012	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176170	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176170	.	start_codon	1040	1040	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176170	.	non_covalent_binding_site	1041	1059	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176170	.	engineered_region	988	1040	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
>BBa_K176170
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggatt
cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccc
tttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcag
tttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccgg
ggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgca
tatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggct
gatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaact
acgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata