LOCUS       BBa_K176183             1950 bp    DNA     linear       10-May-2024
DEFINITION  aTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS
ACCESSION   BBa_K176183
VERSION     BBa_K176183.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23103
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     misc_feature    44..746
                     /label=BBa_S0101
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    44..883
                     /label=BBa_K176006
     promoter        892..926
                     /label=BBa_J23109
     misc_feature    939..942
                     /label=conserved
     RBS             935..946
                     /label=BBa_B0034
     misc_feature    953..954
                     /label=prefix
     misc_feature    1126..1126
                     /label=T
     misc_feature    1444..1444
                     /label=A
     CDS             953..1702
                     /label=luxR
     misc_feature    953..1708
                     /label=BBa_C0062
     misc_feature    1709..1733
                     /label=Help:Barcodes
     CDS             953..1708
                     /label=BBa_C0062
     stem_loop       1753..1796
                     /label=stem_loop
     misc_feature    1742..1821
                     /label=BBa_B0010
     terminator      1742..1821
                     /label=BBa_B0010
     stem_loop       1837..1856
                     /label=T7 TE
     misc_feature    1863..1863
                     /label=stop
     polyA_site      1857..1870
                     /label=polya
     misc_feature    1830..1870
                     /label=BBa_B0012
     terminator      1830..1870
                     /label=BBa_B0012
     misc_feature    935..1870
                     /label=BBa_I0462
     misc_feature    1879..1931
                     /label=R0062
     promoter        1898..1903
                     /label=-35
     promoter        1920..1925
                     /label=-10
     misc_feature    1932..1950
                     /label=tetR
     misc_feature    1931..1931
                     /label=+1
     misc_feature    1879..1898
                     /label=luxR/HSL
     promoter        1879..1950
                     /label=BBa_K176000
     misc_feature    935..1950
                     /label=BBa_K176123
     misc_feature    892..1950
                     /label=BBa_K176129
     misc_feature    44..1950
                     /label=BBa_K176173
ORIGIN
        1 ctgatagcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtttacagct
      901 agctcagtcc tagggactgt gctagctact agagaaagag gagaaatact agatgaaaaa
      961 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa
     1021 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact
     1081 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc
     1141 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga
     1201 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa
     1261 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag
     1321 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa
     1381 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc
     1441 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt
     1501 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat
     1561 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat
     1621 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat
     1681 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga
     1741 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1801 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1861 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt
     1921 atagtcgaat atccctatca gtgatagaga
//