BBa_K176190
1
AID
Activation-Induced (Cytidine) Deaminase (standard version of K103001)
2009-10-09T11:00:00Z
2015-05-08T01:11:04Z
PCR from [[Part:BBa_K103001|K103001]].
Forward Primer: GTTTC T TCTAGa tg gacagcctcttgatgaacc
Reverse Primer: GTTTC CTGCA G CGGCCGC T ACTAGT A TTATTa aagtcccaaagtacgaaatg
Digest with XbaI and PstI, ligate into pSB1A3.
AID is an enzyme which can increase mutation rate in vivo by C->U conversion on DNA.
[[Part:BBa_K103001|K103001]] is the coding sequence of AID with NcoI site before start codon. This part K176190 is a standard AID coding sequence starts with ATG and ends with TAATAA.
Reference for Biology and Usage:
http://en.wikipedia.org/wiki/Activation-Induced_%28Cytidine%29_Deaminase
http://2009.igem.org/Team:USTC
http://2008.igem.org/Team:Warsaw
http://2008.igem.org/Team:Peking_University
http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Gamma_-_automatic_directed_evolution
false
false
_282_
0
3908
9
It's complicated
true
Standard AID coding sequence starts with ATG and ends with TAATAA.
false
Danqian Liu, Zongxiao He, Hao Jiang
annotation2032941
1
Activation-Induced (Cytidine) Deaminase
range2032941
1
1
594
annotation2032940
1
Stop
range2032940
1
595
600
annotation2032939
1
Start
range2032939
1
1
3
BBa_K176191
1
BBa_K176191
PoPS->RBS(weak)+AID->PoPS
2009-10-09T11:00:00Z
2015-05-08T01:11:04Z
B0031 K176190
Weak RBS(B0031) + Activation-Induced (Cytidine) Deaminase coding sequence
false
false
_282_
0
3908
9
It's complicated
false
Weak RBS(B0031) + Activation-Induced (Cytidine) Deaminase coding sequence
false
Danqian Liu, Zongxiao He, Hao Jiang
component2032943
1
BBa_B0031
component2032947
1
BBa_K176190
annotation2032943
1
BBa_B0031
range2032943
1
1
14
annotation2032947
1
BBa_K176190
range2032947
1
21
620
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_K176191_sequence
1
tcacacaggaaacctactagatggacagcctcttgatgaaccggaggaagtttctttaccaattcaaaaatgtccgctgggctaagggtcggcgtgagacctacctgtgctacgtagtgaagaggcgtgacagtgctacatccttttcactggactttggttatcttcgcaataagaacggctgccacgtggaattgctcttcctccgctacatctcggactgggacctagaccctggccgctgctaccgcgtcacctggttcacctcctggagcccctgctacgactgtgcccgacatgtggccgactttctgcgagggaaccccaacctcagtctgaggatcttcaccgcgcgcctctacttctgtgaggaccgcaaggctgagcccgaggggctgcggcggctgcaccgcgccggggtgcaaatagccatcatgaccttcaaagattatttttactgctggaatacttttgtagaaaaccatgaaagaactttcaaagcctgggaagggctgcatgaaaattcagttcgtctctccagacagcttcggcgcatccttttgcccctgtatgaggttgatgacttacgagacgcatttcgtactttgggactttaataa
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_K176190_sequence
1
atggacagcctcttgatgaaccggaggaagtttctttaccaattcaaaaatgtccgctgggctaagggtcggcgtgagacctacctgtgctacgtagtgaagaggcgtgacagtgctacatccttttcactggactttggttatcttcgcaataagaacggctgccacgtggaattgctcttcctccgctacatctcggactgggacctagaccctggccgctgctaccgcgtcacctggttcacctcctggagcccctgctacgactgtgcccgacatgtggccgactttctgcgagggaaccccaacctcagtctgaggatcttcaccgcgcgcctctacttctgtgaggaccgcaaggctgagcccgaggggctgcggcggctgcaccgcgccggggtgcaaatagccatcatgaccttcaaagattatttttactgctggaatacttttgtagaaaaccatgaaagaactttcaaagcctgggaagggctgcatgaaaattcagttcgtctctccagacagcttcggcgcatccttttgcccctgtatgaggttgatgacttacgagacgcatttcgtactttgggactttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z