LOCUS       BBa_K176192             2402 bp    DNA     linear       22-Dec-2024
DEFINITION  PoPS->GFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPS
ACCESSION   BBa_K176192
VERSION     BBa_K176192.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    1..738
                     /label=BBa_I13500
     misc_feature    751..754
                     /label=conserved
     RBS             747..758
                     /label=BBa_B0034
     CDS             768..1475
                     /label=cI lambda
     misc_feature    1476..1508
                     /label=LVA
     misc_feature    1515..1539
                     /label=Help:Barcodes
     CDS             765..1514
                     /label=BBa_C0051
     stem_loop       1559..1602
                     /label=stem_loop
     misc_feature    1548..1627
                     /label=BBa_B0010
     terminator      1548..1627
                     /label=BBa_B0010
     stem_loop       1643..1662
                     /label=T7 TE
     misc_feature    1669..1669
                     /label=stop
     polyA_site      1663..1676
                     /label=polya
     misc_feature    1636..1676
                     /label=BBa_B0012
     terminator      1636..1676
                     /label=BBa_B0012
     promoter        1722..1727
                     /label=-10
     promoter        1699..1704
                     /label=-35
     misc_feature    1709..1725
                     /label=OR1
     misc_feature    1685..1701
                     /label=OR2
     misc_feature    1685..1733
                     /label=BBa_R0051
     promoter        1685..1733
                     /label=BBa_R0051
     misc_feature    747..1733
                     /label=BBa_Q04510
     misc_feature    1746..1749
                     /label=conserved
     RBS             1742..1753
                     /label=BBa_B0034
     misc_feature    2339..2370
                     /label=LVA
     CDS             1760..2338
                     /label=luxI
     misc_feature    1760..2377
                     /label=BBa_C0061
     misc_feature    2378..2402
                     /label=Help:Barcodes
     CDS             1760..2377
                     /label=BBa_C0061
     misc_feature    1742..2402
                     /label=BBa_C0261
     misc_feature    747..2402
                     /label=BBa_K176189
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagaaag aggagaaata ctagatgagc acaaaaaaga
      781 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa
      841 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt
      901 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc
      961 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct
     1021 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg
     1081 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg
     1141 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg
     1201 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt
     1261 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg
     1321 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac
     1381 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag
     1441 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt
     1501 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat
     1561 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1621 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     1681 agagtaacac cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga
     1741 gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc
     1801 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga
     1861 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga
     1921 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac
     1981 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc
     2041 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat
     2101 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt
     2161 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa
     2221 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac
     2281 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc
     2341 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
     2401 tc
//