LOCUS BBa_K176193 3030 bp DNA linear 19-Dec-2024 DEFINITION PoPS->AID(week RBS)->GFP->CI Inverter (cI-LVA+pCI)->luxI-LVA->PoPS ACCESSION BBa_K176193 VERSION BBa_K176193.1 FEATURES Location/Qualifiers misc_feature 7..10 /label=conserved RBS 1..14 /label=BBa_B0031 misc_feature 21..23 /label=Start misc_feature 615..620 /label=Stop CDS 21..614 /label=Activation-Induced (Cytidine) Deaminase CDS 21..620 /label=BBa_K176190 misc_feature 1..620 /label=BBa_K176191 misc_feature 633..636 /label=conserved RBS 629..640 /label=BBa_B0034 CDS 647..1366 /label=GFP protein CDS 647..1366 /label=BBa_E0040 misc_feature 629..1366 /label=BBa_I13500 misc_feature 1379..1382 /label=conserved RBS 1375..1386 /label=BBa_B0034 CDS 1396..2103 /label=cI lambda misc_feature 2104..2136 /label=LVA misc_feature 2143..2167 /label=Help:Barcodes CDS 1393..2142 /label=BBa_C0051 stem_loop 2187..2230 /label=stem_loop misc_feature 2176..2255 /label=BBa_B0010 terminator 2176..2255 /label=BBa_B0010 stem_loop 2271..2290 /label=T7 TE misc_feature 2297..2297 /label=stop polyA_site 2291..2304 /label=polya misc_feature 2264..2304 /label=BBa_B0012 terminator 2264..2304 /label=BBa_B0012 promoter 2350..2355 /label=-10 promoter 2327..2332 /label=-35 misc_feature 2337..2353 /label=OR1 misc_feature 2313..2329 /label=OR2 misc_feature 2313..2361 /label=BBa_R0051 promoter 2313..2361 /label=BBa_R0051 misc_feature 1375..2361 /label=BBa_Q04510 misc_feature 2374..2377 /label=conserved RBS 2370..2381 /label=BBa_B0034 misc_feature 2967..2998 /label=LVA CDS 2388..2966 /label=luxI misc_feature 2388..3005 /label=BBa_C0061 misc_feature 3006..3030 /label=Help:Barcodes CDS 2388..3005 /label=BBa_C0061 misc_feature 2370..3030 /label=BBa_C0261 misc_feature 1375..3030 /label=BBa_K176189 misc_feature 629..3030 /label=BBa_K176192 ORIGIN 1 tcacacagga aacctactag atggacagcc tcttgatgaa ccggaggaag tttctttacc 61 aattcaaaaa tgtccgctgg gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga 121 agaggcgtga cagtgctaca tccttttcac tggactttgg ttatcttcgc aataagaacg 181 gctgccacgt ggaattgctc ttcctccgct acatctcgga ctgggaccta gaccctggcc 241 gctgctaccg cgtcacctgg ttcacctcct ggagcccctg ctacgactgt gcccgacatg 301 tggccgactt tctgcgaggg aaccccaacc tcagtctgag gatcttcacc gcgcgcctct 361 acttctgtga ggaccgcaag gctgagcccg aggggctgcg gcggctgcac cgcgccgggg 421 tgcaaatagc catcatgacc ttcaaagatt atttttactg ctggaatact tttgtagaaa 481 accatgaaag aactttcaaa gcctgggaag ggctgcatga aaattcagtt cgtctctcca 541 gacagcttcg gcgcatcctt ttgcccctgt atgaggttga tgacttacga gacgcatttc 601 gtactttggg actttaataa tactagagaa agaggagaaa tactagatgc gtaaaggaga 661 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 721 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 781 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 841 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 901 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 961 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 1021 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 1081 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 1141 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 1201 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 1261 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 1321 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagaaagag 1381 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 1441 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 1501 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 1561 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 1621 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg 1681 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 1741 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 1801 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 1861 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 1921 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 1981 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 2041 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt 2101 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 2161 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2221 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2281 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc 2341 tctggcggtg ataatggttg ctactagaga aagaggagaa atactagatg actataatga 2401 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 2461 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 2521 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 2581 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 2641 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 2701 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 2761 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 2821 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 2881 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 2941 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 3001 aataactctg atagtgctag tgtagatctc //