##gff-version 3
##sequence-region BBa_K176193 1 3030
BBa_K176193	.	engineered_region	629	3030	.	+	0	ID=BBa_K176192;Name=BBa_K176192
BBa_K176193	.	engineered_region	1375	3030	.	+	0	ID=BBa_K176189;Name=BBa_K176189;Parent=BBa_K176192
BBa_K176193	.	engineered_region	2370	3030	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176189
BBa_K176193	.	CDS	2388	3005	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176193	.	engineered_region	2388	3005	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176193	.	tag	2967	2998	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176193	.	engineered_tag	3006	3030	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176193	.	CDS	2388	2966	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176193	.	ribosome_entry_site	2370	2381	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176193	.	conserved_region	2374	2377	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176193	.	engineered_region	1375	2361	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_K176189
BBa_K176193	.	terminator	2264	2304	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K176193	.	engineered_region	2264	2304	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176193	.	stop_codon	2297	2297	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176193	.	stem_loop	2271	2290	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176193	.	polyA_site	2291	2304	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176193	.	promoter	2313	2361	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K176193	.	promoter	2327	2332	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K176193	.	promoter	2350	2355	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K176193	.	non_covalent_binding_site	2313	2329	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K176193	.	engineered_region	2313	2361	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K176193	.	non_covalent_binding_site	2337	2353	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K176193	.	terminator	2176	2255	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K176193	.	stem_loop	2187	2230	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176193	.	engineered_region	2176	2255	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176193	.	ribosome_entry_site	1375	1386	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K176193	.	conserved_region	1379	1382	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176193	.	CDS	1393	2142	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K176193	.	tag	2104	2136	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K176193	.	CDS	1396	2103	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K176193	.	engineered_tag	2143	2167	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K176193	.	engineered_region	629	1366	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_K176192
BBa_K176193	.	ribosome_entry_site	629	640	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_K176193	.	conserved_region	633	636	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176193	.	CDS	647	1366	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_K176193	.	CDS	647	1366	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176193	.	engineered_region	1	620	.	+	0	ID=BBa_K176191;Name=BBa_K176191
BBa_K176193	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176191
BBa_K176193	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176193	.	CDS	21	620	.	+	0	ID=BBa_K176190;Name=BBa_K176190;Parent=BBa_K176191
BBa_K176193	.	stop_codon	615	620	.	+	0	ID=annotation2032940;Name=Stop;Parent=BBa_K176190
BBa_K176193	.	CDS	21	614	.	+	0	ID=annotation2032941;Name=Activation-Induced (Cytidine) Deaminase;Parent=BBa_K176190
BBa_K176193	.	start_codon	21	23	.	+	0	ID=annotation2032939;Name=Start;Parent=BBa_K176190
>BBa_K176193
tcacacaggaaacctactagatggacagcctcttgatgaaccggaggaagtttctttaccaattcaaaaatgtccgctg
ggctaagggtcggcgtgagacctacctgtgctacgtagtgaagaggcgtgacagtgctacatccttttcactggacttt
ggttatcttcgcaataagaacggctgccacgtggaattgctcttcctccgctacatctcggactgggacctagaccctg
gccgctgctaccgcgtcacctggttcacctcctggagcccctgctacgactgtgcccgacatgtggccgactttctgcg
agggaaccccaacctcagtctgaggatcttcaccgcgcgcctctacttctgtgaggaccgcaaggctgagcccgagggg
ctgcggcggctgcaccgcgccggggtgcaaatagccatcatgaccttcaaagattatttttactgctggaatacttttg
tagaaaaccatgaaagaactttcaaagcctgggaagggctgcatgaaaattcagttcgtctctccagacagcttcggcg
catccttttgcccctgtatgaggttgatgacttacgagacgcatttcgtactttgggactttaataatactagagaaag
aggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgt
taatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgc
actactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacc
cagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaa
agatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt
attgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatgg
cagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcc
ctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggca
tggatgaactatacaaataataatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaa
gagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctg
tcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgc
cgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtat
gaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttct
cacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcatt
ctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctc
gttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaac
tgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttc
cgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagta
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagaga
aagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtat
tctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgag
tatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattaccta
caacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagt
cgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactattt
gaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttt
taaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtatt
gtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctc