##gff-version 3
##sequence-region BBa_K176200 1 2586
BBa_K176200	.	engineered_region	1	1908	.	+	0	ID=BBa_K176083;Name=BBa_K176083
BBa_K176200	.	engineered_region	1	841	.	+	0	ID=BBa_K176020;Name=BBa_K176020;Parent=BBa_K176083
BBa_K176200	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176020
BBa_K176200	.	engineered_region	44	841	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176020
BBa_K176200	.	terminator	713	841	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176200	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176200	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176200	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176200	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176200	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176200	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176200	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176200	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176200	.	engineered_region	44	704	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176200	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176200	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176200	.	CDS	62	679	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176200	.	CDS	62	640	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176200	.	engineered_tag	680	704	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176200	.	engineered_region	62	679	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176200	.	tag	641	672	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176200	.	engineered_region	850	1908	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176083
BBa_K176200	.	engineered_region	850	1828	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176200	.	promoter	850	884	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176200	.	engineered_region	893	1828	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176200	.	terminator	1788	1828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176200	.	stop_codon	1821	1821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176200	.	polyA_site	1815	1828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176200	.	stem_loop	1795	1814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176200	.	engineered_region	1788	1828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176200	.	CDS	911	1666	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176200	.	engineered_tag	1667	1691	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176200	.	engineered_region	911	1666	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176200	.	engineered_region	911	912	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176200	.	point_mutation	1084	1084	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176200	.	CDS	911	1660	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176200	.	point_mutation	1402	1402	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176200	.	terminator	1700	1779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176200	.	engineered_region	1700	1779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176200	.	stem_loop	1711	1754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176200	.	ribosome_entry_site	893	904	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176200	.	conserved_region	897	900	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176200	.	promoter	1837	1908	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176200	.	promoter	1856	1861	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176200	.	non_covalent_binding_site	1837	1856	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176200	.	promoter	1878	1883	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176200	.	non_covalent_binding_site	1890	1908	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176200	.	start_codon	1889	1889	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176200	.	engineered_region	1837	1889	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176200	.	engineered_region	1917	2586	.	+	0	ID=BBa_K176166;Name=BBa_K176166
BBa_K176200	.	terminator	2458	2537	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176166
BBa_K176200	.	engineered_region	2458	2537	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176200	.	stem_loop	2469	2512	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176200	.	terminator	2546	2586	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176166
BBa_K176200	.	stop_codon	2579	2579	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176200	.	polyA_site	2573	2586	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176200	.	stem_loop	2553	2572	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176200	.	engineered_region	2546	2586	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176200	.	CDS	1937	2449	.	+	0	ID=BBa_K176158;Name=BBa_K176158;Parent=BBa_K176166
BBa_K176200	.	primer_binding_site	1977	1993	.	+	0	ID=annotation2020922;Name=M13 -40 primer;Parent=BBa_K176158
BBa_K176200	.	point_mutation	2191	2191	.	+	0	ID=annotation2020927;Name=c in K145151;Parent=BBa_K176158
BBa_K176200	.	point_mutation	2101	2101	.	+	0	ID=annotation2020923;Name=g and 69bp in I732006;Parent=BBa_K176158
BBa_K176200	.	stop_codon	2444	2449	.	+	0	ID=annotation2020916;Name=Stop;Parent=BBa_K176158
BBa_K176200	.	CDS	1937	2410	.	+	0	ID=annotation2020913;Name=lacZalpha-ccdB;Parent=BBa_K176158
BBa_K176200	.	start_codon	1937	1939	.	+	0	ID=annotation2020918;Name=Start;Parent=BBa_K176158
BBa_K176200	.	engineered_region	1937	2101	.	+	0	ID=annotation2020917;Name=a part of lacZalpha (I732006);Parent=BBa_K176158
BBa_K176200	.	sequence_alteration	1952	1952	.	+	0	ID=annotation2020919;Name=177bp and a in J32026;Parent=BBa_K176158
BBa_K176200	.	primer_binding_site	1977	2000	.	+	0	ID=annotation2020921;Name=M13 -47 primer (K176059);Parent=BBa_K176158
BBa_K176200	.	primer_binding_site	2124	2151	.	+	0	ID=annotation2020926;Name=VRccdB primer (K176058);Parent=BBa_K176158
BBa_K176200	.	primer_binding_site	1957	1973	.	+	0	ID=annotation2020920;Name=M13 -20 primer;Parent=BBa_K176158
BBa_K176200	.	tag	2411	2443	.	+	0	ID=annotation2020914;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176158
BBa_K176200	.	sequence_alteration	2108	2109	.	+	0	ID=annotation2020924;Name=at in K145151;Parent=BBa_K176158
BBa_K176200	.	engineered_region	2108	2410	.	+	0	ID=annotation2020925;Name=ccdB (K145151);Parent=BBa_K176158
BBa_K176200	.	ribosome_entry_site	1917	1930	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176166
BBa_K176200	.	conserved_region	1923	1926	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_K176200
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgactataatgataaaa
aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac
ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg
tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct
gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct
caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat
tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga
gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag
tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcct
aggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataat
taataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaa
tattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaat
ggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaat
taattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctt
atcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagaca
actatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaa
aataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagga
aaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatga
aactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaa
ttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctat
cagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaa
cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaata
gcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataa
aagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatcccc
ctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggc
gcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaa
tgacatcaaaaacgccattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata