LOCUS BBa_K176206 2584 bp DNA linear 22-Nov-2024 DEFINITION Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA ACCESSION BBa_K176206 VERSION BBa_K176206.1 FEATURES Location/Qualifiers promoter 1..6 /label=-35 promoter 24..29 /label=-10 misc_feature 17..17 /label=c in J23115 misc_feature 21..21 /label=t in J23115 misc_feature 7..12 /label=NheI misc_feature 30..35 /label=NheI misc_feature 18..23 /label=AvrII promoter 1..35 /label=BBa_K176008 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 misc_feature 44..704 /label=BBa_C0261 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 terminator 713..841 /label=BBa_B0015 misc_feature 44..841 /label=BBa_K082014 misc_feature 1..841 /label=BBa_K176022 promoter 850..884 /label=BBa_J23101 misc_feature 897..900 /label=conserved RBS 893..904 /label=BBa_B0034 misc_feature 911..912 /label=prefix misc_feature 1084..1084 /label=T misc_feature 1402..1402 /label=A CDS 911..1660 /label=luxR misc_feature 911..1666 /label=BBa_C0062 misc_feature 1667..1691 /label=Help:Barcodes CDS 911..1666 /label=BBa_C0062 stem_loop 1711..1754 /label=stem_loop misc_feature 1700..1779 /label=BBa_B0010 terminator 1700..1779 /label=BBa_B0010 stem_loop 1795..1814 /label=T7 TE misc_feature 1821..1821 /label=stop polyA_site 1815..1828 /label=polya misc_feature 1788..1828 /label=BBa_B0012 terminator 1788..1828 /label=BBa_B0012 misc_feature 893..1828 /label=BBa_I0462 misc_feature 850..1828 /label=BBa_K176025 misc_feature 1837..1889 /label=R0062 promoter 1856..1861 /label=-35 promoter 1878..1883 /label=-10 misc_feature 1890..1908 /label=tetR misc_feature 1889..1889 /label=+1 misc_feature 1837..1856 /label=luxR/HSL promoter 1837..1908 /label=BBa_K176000 misc_feature 850..1908 /label=BBa_K176028 misc_feature 1..1908 /label=BBa_K176085 misc_feature 1921..1924 /label=conserved RBS 1917..1928 /label=BBa_B0034 CDS 1935..2408 /label=lacZalpha-ccdB misc_feature 2409..2441 /label=LVA degradation tag (AANDENYALVA) misc_feature 2442..2447 /label=Stop misc_feature 1935..2099 /label=a part of lacZalpha (I732006) misc_feature 1935..1937 /label=Start misc_feature 1950..1950 /label=177bp and a in J32026 primer_bind 1955..1971 /label=M13 -20 primer primer_bind 1975..1998 /label=M13 -47 primer (K176059) primer_bind 1975..1991 /label=M13 -40 primer misc_feature 2099..2099 /label=g and 69bp in I732006 misc_feature 2106..2107 /label=at in K145151 misc_feature 2106..2408 /label=ccdB (K145151) primer_bind 2122..2149 /label=VRccdB primer (K176058) misc_feature 2189..2189 /label=c in K145151 CDS 1935..2447 /label=BBa_K176158 stem_loop 2467..2510 /label=stem_loop misc_feature 2456..2535 /label=BBa_B0010 terminator 2456..2535 /label=BBa_B0010 stem_loop 2551..2570 /label=T7 TE misc_feature 2577..2577 /label=stop polyA_site 2571..2584 /label=polya misc_feature 2544..2584 /label=BBa_B0012 terminator 2544..2584 /label=BBa_B0012 misc_feature 1917..2584 /label=BBa_K176164 ORIGIN 1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagagt ttacagctag ctcagtccta ggtattatgc tagctactag agaaagagga 901 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa 961 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca 1021 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc 1081 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa 1141 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt 1201 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg 1261 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag 1321 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa 1381 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa 1441 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg 1501 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca 1561 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc 1621 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta 1681 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1801 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 1861 gcaagaaaat ggtttgttat agtcgaatat ccctatcagt gatagagata ctagagaaag 1921 aggagaaata ctagatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg 1981 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 2041 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat 2101 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 2161 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg 2221 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 2281 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 2341 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 2401 ggggaatagc tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg 2461 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2521 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2581 tata //