##gff-version 3
##sequence-region BBa_K176213 1 2413
BBa_K176213	.	engineered_region	1	1908	.	+	0	ID=BBa_K176087;Name=BBa_K176087
BBa_K176213	.	engineered_region	850	1908	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176087
BBa_K176213	.	promoter	1837	1908	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176213	.	promoter	1878	1883	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176213	.	promoter	1856	1861	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176213	.	start_codon	1889	1889	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176213	.	non_covalent_binding_site	1837	1856	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176213	.	non_covalent_binding_site	1890	1908	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176213	.	engineered_region	1837	1889	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176213	.	engineered_region	850	1828	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176213	.	promoter	850	884	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176213	.	engineered_region	893	1828	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176213	.	terminator	1700	1779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176213	.	stem_loop	1711	1754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176213	.	engineered_region	1700	1779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176213	.	ribosome_entry_site	893	904	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176213	.	conserved_region	897	900	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176213	.	CDS	911	1666	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176213	.	CDS	911	1660	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176213	.	engineered_region	911	1666	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176213	.	engineered_region	911	912	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176213	.	point_mutation	1084	1084	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176213	.	engineered_tag	1667	1691	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176213	.	point_mutation	1402	1402	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176213	.	terminator	1788	1828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176213	.	stop_codon	1821	1821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176213	.	engineered_region	1788	1828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176213	.	stem_loop	1795	1814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176213	.	polyA_site	1815	1828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176213	.	engineered_region	1	841	.	+	0	ID=BBa_K176024;Name=BBa_K176024;Parent=BBa_K176087
BBa_K176213	.	engineered_region	44	841	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176024
BBa_K176213	.	engineered_region	44	704	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176213	.	CDS	62	679	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176213	.	engineered_tag	680	704	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176213	.	engineered_region	62	679	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176213	.	CDS	62	640	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176213	.	tag	641	672	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176213	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176213	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176213	.	terminator	713	841	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176213	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176213	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176213	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176213	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176213	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176213	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176213	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176213	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176213	.	promoter	1	35	.	+	0	ID=BBa_J23103;Name=BBa_J23103;Parent=BBa_K176024
BBa_K176213	.	engineered_region	1917	2413	.	+	0	ID=BBa_K176163;Name=BBa_K176163
BBa_K176213	.	ribosome_entry_site	1917	1928	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176163
BBa_K176213	.	conserved_region	1921	1924	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176213	.	terminator	2285	2364	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176163
BBa_K176213	.	stem_loop	2296	2339	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176213	.	engineered_region	2285	2364	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176213	.	terminator	2373	2413	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176163
BBa_K176213	.	stop_codon	2406	2406	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176213	.	engineered_region	2373	2413	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176213	.	stem_loop	2380	2399	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176213	.	polyA_site	2400	2413	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176213	.	CDS	1935	2276	.	+	0	ID=BBa_K176157;Name=BBa_K176157;Parent=BBa_K176163
BBa_K176213	.	tag	2238	2270	.	+	0	ID=annotation2020909;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176157
BBa_K176213	.	start_codon	1935	1937	.	+	0	ID=annotation2020910;Name=Start;Parent=BBa_K176157
BBa_K176213	.	stop_codon	2271	2276	.	+	0	ID=annotation2020911;Name=Stop;Parent=BBa_K176157
BBa_K176213	.	CDS	1935	2237	.	+	0	ID=annotation2020908;Name=ccdB (K145151);Parent=BBa_K176157
BBa_K176213	.	primer_binding_site	1951	1978	.	+	0	ID=annotation2020912;Name=VRccdB primer (K176058);Parent=BBa_K176157
>BBa_K176213
ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgactataatgataaaa
aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac
ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg
tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct
gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct
caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat
tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga
gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag
tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcct
aggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataat
taataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaa
tattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaat
ggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaat
taattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctt
atcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagaca
actatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaa
aataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagga
aaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatga
aactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaa
ttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctat
cagtgatagagatactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgtta
tcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgt
ctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccg
atatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgc
cattaacctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata