LOCUS       BBa_K176224             2388 bp    DNA     linear       26-Jan-2026
DEFINITION  PoPS->mRFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPS
ACCESSION   BBa_K176224
VERSION     BBa_K176224.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..699
                     /label=BBa_E1010
     misc_feature    1..724
                     /label=BBa_I13502
     misc_feature    737..740
                     /label=conserved
     RBS             733..744
                     /label=BBa_B0034
     CDS             754..1461
                     /label=cI lambda
     misc_feature    1462..1494
                     /label=LVA
     misc_feature    1501..1525
                     /label=Help:Barcodes
     CDS             751..1500
                     /label=BBa_C0051
     stem_loop       1545..1588
                     /label=stem_loop
     misc_feature    1534..1613
                     /label=BBa_B0010
     terminator      1534..1613
                     /label=BBa_B0010
     stem_loop       1629..1648
                     /label=T7 TE
     misc_feature    1655..1655
                     /label=stop
     polyA_site      1649..1662
                     /label=polya
     misc_feature    1622..1662
                     /label=BBa_B0012
     terminator      1622..1662
                     /label=BBa_B0012
     promoter        1708..1713
                     /label=-10
     promoter        1685..1690
                     /label=-35
     misc_feature    1695..1711
                     /label=OR1
     misc_feature    1671..1687
                     /label=OR2
     misc_feature    1671..1719
                     /label=BBa_R0051
     promoter        1671..1719
                     /label=BBa_R0051
     misc_feature    733..1719
                     /label=BBa_Q04510
     misc_feature    1732..1735
                     /label=conserved
     RBS             1728..1739
                     /label=BBa_B0034
     misc_feature    2325..2356
                     /label=LVA
     CDS             1746..2324
                     /label=luxI
     misc_feature    1746..2363
                     /label=BBa_C0061
     misc_feature    2364..2388
                     /label=Help:Barcodes
     CDS             1746..2363
                     /label=BBa_C0061
     misc_feature    1728..2388
                     /label=BBa_C0261
     misc_feature    733..2388
                     /label=BBa_K176189
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa
      781 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt
      841 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct
      901 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc
      961 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa
     1021 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt
     1081 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg
     1141 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc
     1201 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac
     1261 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt
     1321 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag
     1381 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag
     1441 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa
     1501 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
     1561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1621 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg
     1681 cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat
     1741 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata
     1801 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg
     1861 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt
     1921 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata
     1981 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata
     2041 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta
     2101 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta
     2161 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc
     2221 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat
     2281 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa
     2341 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctc
//